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Literature summary for 3.2.1.1 extracted from

  • Ikawa, K.; Araki, H.; Tsujino, Y.; Hayashi, Y.; Igarashi, K.; Hatada, Y.; Hagihara, H.; Ozawa, T.; Ozaki, K.; Kobayashi, T.; Ito, S.
    Hyperexpression of the gene for a Bacillus alpha-amylase in Bacillus subtilis cells: enzymatic properties and crystallization of the recombinant enzyme (1998), Biosci. Biotechnol. Biochem., 62, 1720-1725.
    View publication on PubMed
Search for more literature for 3.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
hyperexpression in Bacillus subtilis Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant enzyme Bacillus sp. (in: Bacteria)

General Stability

General Stability Organism
Ca2+, 0.5 mM, and Na+, 12.5 mM, completely protect from thermoinactivation at 60°C Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
EDTA no effect Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ catalytic and/or structure-stabilizing Ca2+ ions are tightly bound to the enzyme Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 x * 53000, recombinant enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) KSM-1378
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
 Bacillus sp. (in: Bacteria) additional information
-
 ?
amylopectin + H2O
-
 Bacillus sp. (in: Bacteria) KSM-1378 additional information
-
 ?
amylose + H2O
-
 Bacillus sp. (in: Bacteria) additional information
-
 ?
amylose + H2O
-
 Bacillus sp. (in: Bacteria) KSM-1378 additional information
-
 ?
maltodextrin + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
maltodextrin + H2O
-
 Bacillus sp. (in: Bacteria) KSM-1378 ?
-
 ?
starch + H2O soluble starch Bacillus sp. (in: Bacteria) additional information the major products are maltotriose + maltopentaose ?
starch + H2O soluble starch Bacillus sp. (in: Bacteria) KSM-1378 additional information the major products are maltotriose + maltopentaose ?

Subunits

Subunits Comment Organism
? x * 53000, recombinant enzyme, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
LAMY
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55 60 recombinant enzyme Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 20 min, recombinant enzyme, stable up to Bacillus sp. (in: Bacteria)
60
-
 20 min, recombinant enzyme, complete loss of activity, Ca2+, 0.5 mM, and Na+, 12.5 mM, completely protect from thermoinactivation Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 recombinant enzyme Bacillus sp. (in: Bacteria)