Any feedback?
Literature summary for 3.2.1.1 extracted from
- Purification, properties and structural aspects of a thermoacidophilic alpha-amylase from Alicyclobacillus acidocaldarius ATCC 27009. Insight into acidostability of proteins (1994), Eur. J. Biochem., 226, 981-991.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Alicyclobacillus acidocaldarius |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Alicyclobacillus acidocaldarius | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
x * 160000, SDS-PAGE | Alicyclobacillus acidocaldarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Alicyclobacillus acidocaldarius | - |
ATCC 27009 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate is O-glycosidically linked to the protein, the carbohydrate moiety is confined to the region N-terminal to Asp466 or C-terminal to approximately residue 1200 | Alicyclobacillus acidocaldarius |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Alicyclobacillus acidocaldarius |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Alicyclobacillus acidocaldarius | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.9 | - |
- |
Alicyclobacillus acidocaldarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pullulan + H2O | - |
Alicyclobacillus acidocaldarius | panose + ? | - |
? |  |
| starch + H2O | - |
Alicyclobacillus acidocaldarius | additional information | maltotriose + maltose, glucose is also formed | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 160000, SDS-PAGE | Alicyclobacillus acidocaldarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Alicyclobacillus acidocaldarius |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 80 | 60°C: about 40% of maximal activity, 80°C: about 90% of maximal activity, 85°C: about 20% of maximal activity | Alicyclobacillus acidocaldarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
- |
Alicyclobacillus acidocaldarius |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 1.2 | 6 | pH 1.2: about 40% of maximal activity, pH 6.0: about 50% of maximal activity | Alicyclobacillus acidocaldarius |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
