Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and Bacillus subtilis | Pyrococcus furiosus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | slight | Pyrococcus furiosus | |
Cu2+ | 5 mM, complete inhibition | Pyrococcus furiosus | |
Fe2+ | slight | Pyrococcus furiosus | |
Ni2+ | slight inhibition | Pyrococcus furiosus | |
Zn2+ | 5 mM, complete inhibition | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Pyrococcus furiosus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52875 | - |
x * 52875, extracellular enzyme, calculation from nucleotide sequence | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | O08452 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Pyrococcus furiosus | additional information | - |
? | |
amylose + H2O | - |
Pyrococcus furiosus | additional information | - |
? | |
maltodextran + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
maltodextrin + H2O | white dextrin | Pyrococcus furiosus | ? | - |
? | |
starch + H2O | soluble starch | Pyrococcus furiosus | additional information | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52875, extracellular enzyme, calculation from nucleotide sequence | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
- |
Pyrococcus furiosus |
100 | 105 | - |
Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 130 | 70°C: about 55% of maximal activity, 130°C: about 65% of maximal activity | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
6 h, 20% loss of activity | Pyrococcus furiosus |
115 | - |
3 h, 65% loss of activity | Pyrococcus furiosus |
120 | - |
1 h, 40% loss of activity | Pyrococcus furiosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Pyrococcus furiosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7.5 | pH 3.5: about 70% of maximal activity, pH 7.5: about 40% of maximal activity | Pyrococcus furiosus |