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Literature summary for 3.2.1.1 extracted from
- Membrane-bound and soluble extracellular alpha-amylase from Bacillus subtilis (1979), J. Biol. Chem., 254, 8540-8547.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | 1 mM, 39% loss of activity of the membrane-bound enzyme | Bacillus subtilis |  |
| diisopropyl fluorophosphate | 1 mM, 34% inhibition of the membrane-bound enzyme | Bacillus subtilis | |
| EDTA | 10 mM, 67% inhibition | Bacillus subtilis | |
| EGTA | 10 mM, 53% inhibition of the membrane-bound enzyme | Bacillus subtilis | |
| N-alpha-p-tosyl-L-lysine chloromethyl ketone | 1 mM, 22% loss of the activity of the membrane-bound enzyme | Bacillus subtilis | |
| PMSF | 1 mM, 14% loss of activity of the membrane-bound enzyme | Bacillus subtilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus subtilis | - |
- |
| membrane | bound to, the enzyme is latent in membrane vesicles | Bacillus subtilis | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 40 mM calcium acetate, enhances activity of membrane-bound enzyme to 268% of control | Bacillus subtilis | |
| Mg2+ | 40 mM magnesium acetate, enhances activity of the membrane-bound enzyme to 216% of the control | Bacillus subtilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
extracellular enzyme, gel filtration | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
Marburg strain | - |
| Bacillus subtilis Marburg / DSM 10 / ATCC 6051 | - |
Marburg strain | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | membrane-derived enzyme contains phospholipid, predominantly phosphatidylethanolamine | Bacillus subtilis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| extracellular and membrane-bound enzyme | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bacillus subtilis |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 67000, extracellular enzyme, SDS-PAGE | Bacillus subtilis |
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