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Literature summary for 3.2.1.1 extracted from

  • Tanaka, T.; Ishimoto, E.; Shimomura, Y.; Taniguchi, M.; Oi, S.
    Purification and some properties of raw starch-binding amylase of Clostridium butyricum T-7 isolated from mesophilic methane sludge (1987), Agric. Biol. Chem., 51, 399-405.
No PubMed abstract available
Search for more literature for 3.2.1.1

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Clostridium butyricum
Fe2+
-
 Clostridium butyricum
Hg2+ 2 mM, complete inhibition Clostridium butyricum
Mg2+
-
 Clostridium butyricum
Mn2+
-
 Clostridium butyricum
PCMB 0.2 mM, partial Clostridium butyricum
Zn2+
-
 Clostridium butyricum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89000
-
 x * 89000, SDS-PAGE Clostridium butyricum

Organism

Organism UniProt Comment Textmining
Clostridium butyricum
-
-
-
Clostridium butyricum T-7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Clostridium butyricum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O 94% of the activity with starch Clostridium butyricum additional information
-
 ?
amylopectin + H2O 94% of the activity with starch Clostridium butyricum T-7 additional information
-
 ?
amylose + H2O 34% of activity with starch Clostridium butyricum additional information
-
 ?
amylose + H2O 34% of activity with starch Clostridium butyricum T-7 additional information
-
 ?
glycogen + H2O 86% of the activity with starch Clostridium butyricum additional information
-
 ?
glycogen + H2O 86% of the activity with starch Clostridium butyricum T-7 additional information
-
 ?
starch + H2O
-
 Clostridium butyricum additional information major products are maltotriose and maltose ?
starch + H2O
-
 Clostridium butyricum T-7 additional information major products are maltotriose and maltose ?

Subunits

Subunits Comment Organism
? x * 89000, SDS-PAGE Clostridium butyricum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Clostridium butyricum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 65 30°C: about 30% of maximal activity, 65°C: about 35% of maximal activity Clostridium butyricum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 1 h, in absence of Ca2+, stable below Clostridium butyricum
40
-
 1 h, stable below in presence of 1 mM Ca2+ Clostridium butyricum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Clostridium butyricum

pH Range

pH Minimum pH Maximum Comment Organism
3.5 7 about 40% of maximal activity at pH 3.5 and at pH 7.0 Clostridium butyricum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 37°C, 1 h, about 60% loss of activity Clostridium butyricum
5.5 7.5 37°C, 1 h, stable Clostridium butyricum
8
-
 37°C, 1 h, about 75% loss of activity Clostridium butyricum