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Literature summary for 3.13.1.3 extracted from

  • Watkins, L.M.; Rodriguez, R.; Schneider, D.; Broderick, R.; Cruz, M.; Chambers, R.; Ruckman, E.; Cody, M.; Mrachko, G.T.
    Purification and characterization of the aromatic desulfinase, 2-(2'-hydroxyphenyl)benzenesulfinate desulfinase (2003), Arch. Biochem. Biophys., 415, 14-23.
    View publication on PubMed
Search for more literature for 3.13.1.3

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ 10% activation at 0.1 mM Rhodococcus erythropolis
additional information no activation by metal ions Rhodococcus erythropolis

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 18% inhibition at 0.1 mM Rhodococcus erythropolis
Cu2+ 95% inhibition at 0.1 mM Rhodococcus erythropolis
DTNB complete inhibition at 0.01 mM after 10 min Rhodococcus erythropolis
Fe2+ 11% inhibition at 0.1 mM Rhodococcus erythropolis
additional information no product inhibition, no inhibition by Mn2+, Mg2+, and Ca2 Rhodococcus erythropolis
N-bromosuccimide complete inhibition at 0.01 mM after 10 min Rhodococcus erythropolis
phenylglyoxal hydrate 19% inhibition at 0.01 mM after 10 min Rhodococcus erythropolis
pyridoxal-5-phosphate 38% inhibition at 0.01 mM after 10 min Rhodococcus erythropolis
Tetranitromethane complete inhibition at 0.01 mM after 10 min Rhodococcus erythropolis
Zn2+ 50% inhibition at 0.1 mM Rhodococcus erythropolis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 2-(2'-hydroxyphenyl)benzenesulfinate pH 7.4, 35°C Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, SDS-PAGE Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-(2'-hydroxyphenyl)benzenesulfinate + H2O Rhodococcus erythropolis final step of the desulfurozation of dibenzothiophene 2-hydroxybiphenyl + sulfite
-
 ?
2-(2'-hydroxyphenyl)benzenesulfinate + H2O Rhodococcus erythropolis IGTS8 / ATCC 53968 final step of the desulfurozation of dibenzothiophene 2-hydroxybiphenyl + sulfite
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
-
-
Rhodococcus erythropolis IGTS8 / ATCC 53968
-
-
-

Purification (Commentary)

Purification (Comment) Organism
1600fold Rhodococcus erythropolis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00046
-
 purified enzyme Rhodococcus erythropolis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-(2'-hydroxyphenyl)benzenesulfinate + H2O specific for Rhodococcus erythropolis 2-hydroxybiphenyl + sulfite
-
 ?
2-(2'-hydroxyphenyl)benzenesulfinate + H2O final step of the desulfurozation of dibenzothiophene Rhodococcus erythropolis 2-hydroxybiphenyl + sulfite
-
 ?
2-(2'-hydroxyphenyl)benzenesulfinate + H2O specific for Rhodococcus erythropolis IGTS8 / ATCC 53968 2-hydroxybiphenyl + sulfite
-
 ?
2-(2'-hydroxyphenyl)benzenesulfinate + H2O final step of the desulfurozation of dibenzothiophene Rhodococcus erythropolis IGTS8 / ATCC 53968 2-hydroxybiphenyl + sulfite
-
 ?
additional information no activity with xanthene, 2-hydroxydiphenylmetane, 2-methoxybiphenyl, phenylhydroquinone, 2-biphenyl carboxylic acid, 2-amino-4-phenylphenol Rhodococcus erythropolis ?
-
 ?
additional information no activity with xanthene, 2-hydroxydiphenylmetane, 2-methoxybiphenyl, phenylhydroquinone, 2-biphenyl carboxylic acid, 2-amino-4-phenylphenol Rhodococcus erythropolis IGTS8 / ATCC 53968 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Rhodococcus erythropolis

Synonyms

Synonyms Comment Organism
2-(2'-hydroxyphenyl)benzenesulfinate desulfinase
-
 Rhodococcus erythropolis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Rhodococcus erythropolis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 rapid loss of activity at temperatures higher or lower than the optimum Rhodococcus erythropolis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Rhodococcus erythropolis

pH Range

pH Minimum pH Maximum Comment Organism
6 8.4 activity decreases sigificantly below pH 6.0 and above pH 8.4 Rhodococcus erythropolis

pI Value

Organism Comment pI Value Maximum pI Value
Rhodococcus erythropolis isoelectric focusing 5.9 5.6