Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sulfolobus acidocaldarius |
gene agl3, DNA sequencing and analysis of gene agl3 as part of a gene cluster involved in the biosynthesis of sulfoquinovose and important for the assembly of the S-layer N-glycans, expression in Sulfolobus solfataricus agl3 deletion mutant under the control of a maltose inducible promoter using vector pSVA1450, the expression of agl3 leads to the restoration of the production of full-length glycan | Sulfolobus acidocaldarius |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a agl3DELTA deletion mutant strain, that shows a significant change in the size of the S-layer SlaA and the flagellin FlaB glycoproteins, with a changed SlaA glycan composition, phenotype, overview. Change in the phenotype under environmental stress conditions | Sulfolobus acidocaldarius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + sulfite | Sulfolobus acidocaldarius | - |
UDP-alpha-D-sulfoquinovopyranose + H2O | - |
? | |
UDP-alpha-D-glucose + sulfite | Sulfolobus acidocaldarius | the enzyme plays an important role in building up the glycans, which are attached to the S-layer and the flagellin proteins | UDP-alpha-D-sulfoquinovopyranose + H2O | - |
? | |
UDP-alpha-D-glucose + sulfite | Sulfolobus acidocaldarius MW001 | - |
UDP-alpha-D-sulfoquinovopyranose + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | - |
gene agl3, or Saci0423, of the sulfoquinovose biosynthesis operon | - |
Sulfolobus acidocaldarius | Q4JBJ3 | - |
- |
Sulfolobus acidocaldarius MW001 | - |
gene agl3, or Saci0423, of the sulfoquinovose biosynthesis operon | - |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + sulfite | - |
Sulfolobus acidocaldarius | UDP-alpha-D-sulfoquinovopyranose + H2O | - |
? | |
UDP-alpha-D-glucose + sulfite | the enzyme plays an important role in building up the glycans, which are attached to the S-layer and the flagellin proteins | Sulfolobus acidocaldarius | UDP-alpha-D-sulfoquinovopyranose + H2O | - |
? | |
UDP-alpha-D-glucose + sulfite | - |
Sulfolobus acidocaldarius MW001 | UDP-alpha-D-sulfoquinovopyranose + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Agl3 | - |
Sulfolobus acidocaldarius |
Saci_0423 | - |
Sulfolobus acidocaldarius |
General Information | Comment | Organism |
---|---|---|
malfunction | in-frame deletion of agl3 results in a decreased molecular mass of the S-layer glycoprotein SlaA and the flagellin FlaB, indicating a change in the N-glycan composition with reduced trisaccharide structure composed of Man1GlcNAc2, missing the sulfoquinovose, a mannose and glucose. Cells lacking agl3 had a significantly lower growth rate at elevated salt concentrations compared with the background strain, overview | Sulfolobus acidocaldarius |
metabolism | the enzyme is critical for biosynthesis of UDP-sulfoquinovose | Sulfolobus acidocaldarius |