Any feedback?
Literature summary for 3.13.1.1 extracted from
- Native uridine 5'-diphosphate-sulfoquinovose synthase, SQD1, from spinach purified as a 250-kDa complex (2003), Arch. Biochem. Biophys., 413, 123-130.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA isolation and functional expression in Escherichia coli | Spinacia oleracea |
| expression in Escherichia coli | Spinacia oleracea |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km values for UDP-glucose are indistinguishable for the native and the recombinant enzyme, but the Km value for sulfite is over 4fold lower for the native enzyme | Spinacia oleracea |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
| chloroplast | native SoSQD1 is primarily present in the stroma | Spinacia oleracea | 9507 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46700 | - |
x * 46700, calculated from sequence | Spinacia oleracea |
| 250000 | - |
gel filtration | Spinacia oleracea |
| 250000 | - |
native enzyme in a complex with accessory proteins, gel filtration | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + sulfite | Spinacia oleracea | key enzyme in formation of the sulfolipid head group precursor UDP-sulfoquinovose, sulfoquinovosyldiacylglycerol is a polar lipid present in photosynthetic membranes, enzyme is complexed in vivo with accessory proteins | UDP-6-sulfoquinovose + H2O | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | Q84KI6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
| native enzyme from chloroplasts, recombinant enzyme from Escherichia coli | Spinacia oleracea |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite | required NAD+ appears to oxidize the substrate to UDP-4-dehydroglucose, which dehydrates to UDP-4-dehydro-6-deoxygluc-5-enose, to which sulfite can add, the reaction is completed when the substrate is rehydrogenated at C-4 | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + sulfite | - |
Spinacia oleracea | UDP-6-sulfoquinovose + H2O | - |
? | |
| UDP-glucose + sulfite | key enzyme in formation of the sulfolipid head group precursor UDP-sulfoquinovose, sulfoquinovosyldiacylglycerol is a polar lipid present in photosynthetic membranes, enzyme is complexed in vivo with accessory proteins | Spinacia oleracea | UDP-6-sulfoquinovose + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46700, calculated from sequence | Spinacia oleracea |
| dimer | homodimer | Spinacia oleracea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SoSQD1 | - |
Spinacia oleracea |
| SQD1 | - |
Spinacia oleracea |
| sulfite:UDP-glucose sulfotransferase | - |
Spinacia oleracea |
| UDPsulfoquinovose synthase | - |
Spinacia oleracea |
| uridine 5'-diphosphate-sulfoquinovose synthase | - |
Spinacia oleracea |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
