Literature summary for 3.13.1.1 extracted from

Mulichak, A.M.; Theisen, M.J.; Essigmann, B.; Benning, C.; Garavito, R.M.
Crystal structure of SQD1, an enzyme involved in the biosynthesis of the plant sufolipid headgroup donor UDP-sulfoquinovose (1999), Proc. Natl. Acad. Sci. USA, 96, 13097-13102.

Search for more literature for 3.13.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion results in bipyramidal crystals, C-2-symmetric homodimer, the protein has a bidomain structure, catalytic site is located in the cleft formed between the two lobes	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	the function of different amino acids in reaction/binding process is analysed, Thr-145, Tyr-182 and Lys-186 are found to fulfill analogous mechanistic roles	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	thylakoid	Arabidopsis thaliana	9507	-
membrane	photosynthetic	Arabidopsis thaliana	16020	-
thylakoid	-	Arabidopsis thaliana	9579	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-glucose + sulfite	Arabidopsis thaliana	sulfolipid biosynthesis	UDP-sulfoquinovose + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	O48917	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-nitrilotriacetic acid column	Arabidopsis thaliana

Reaction

Reaction	Comment	Organism	Reaction ID
UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite	contains characteristic conserved Y-XXX-K and glycine-rich sequence patterns	Arabidopsis thaliana	a
UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite	short-chain dehydrogenase/reductase family	Arabidopsis thaliana	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-glucose + sulfite	-	Arabidopsis thaliana	UDP-sulfoquinovose + H2O	-	?
UDP-glucose + sulfite	sulfolipid biosynthesis	Arabidopsis thaliana	UDP-sulfoquinovose + H2O	-	?

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)