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show all sequences of 3.11.1.3

Initial in vitro characterisation of phosphonopyruvate hydrolase, a novel phosphate starvation-independent, carbon-phosphorus bond cleavage enzyme in Burkholderia cepacia Pal6

Ternan, N.G.; Hamilton, J.T.; Quinn, J.P.; Arch. Microbiol. 173, 35-41 (2000)

Data extracted from this reference:

General Stability
General Stability
Organism
dialysis of cell-free extract for 24 h at 4C results in a 72% loss of activity
Burkholderia cepacia
Inhibitors
Inhibitors
Commentary
Organism
Structure
3-phosphonopropionic acid
10 mM, 21% inhibition
Burkholderia cepacia
Aminomethylphosphonic acid
10 mM, 19% inhibition
Burkholderia cepacia
EDTA
Co2+, Ni2+, Mg2+, Zn2+, Fe3+ and Ca2+ recover activity. Cu2+, Mn2+ and Cs+ do not recover activity
Burkholderia cepacia
Fe2+
5 mM, 94% inhibition
Burkholderia cepacia
glyphosate
10 mM, 23% inhibition
Burkholderia cepacia
Mn2+
5 mM, 12% inhibition
Burkholderia cepacia
Phosphonoacetate
10 mM, 30% inhibition
Burkholderia cepacia
phosphonobutyric acid
10 mM, 14% inhibition
Burkholderia cepacia
Phosphonoformate
10 mM, 37% inhibition
Burkholderia cepacia
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.72
-
3-phosphonopyruvate
-
Burkholderia cepacia
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
5 mM, 1.5fold activation
Burkholderia cepacia
Co2+
5 mM, 4.8fold activation
Burkholderia cepacia
Cu2+
5 mM, 1.2fold activation
Burkholderia cepacia
Mg2+
5 mM, 3.4fold activation
Burkholderia cepacia
Ni2+
5 mM, 4.3fold activation
Burkholderia cepacia
Zn2+
5 mM, 1.8fold activation
Burkholderia cepacia
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
232000
-
non-denaturing PAGE
Burkholderia cepacia
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-phosphonopyruvate + H2O
Burkholderia cepacia
the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction
pyruvate + phosphate
-
-
?
3-phosphonopyruvate + H2O
Burkholderia cepacia Pal6
the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction
pyruvate + phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Burkholderia cepacia
-
-
-
Burkholderia cepacia Pal6
-
-
-
Storage Stability
Storage Stability
Organism
-20C, 10 weeks, 54% loss of activity
Burkholderia cepacia
20, 50% loss of activity after 3 days, complete loss of activity after 6 days
Burkholderia cepacia
4C, 30 days, 38% loss of activity
Burkholderia cepacia
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-phosphonopyruvate + H2O
-
667335
Burkholderia cepacia
pyruvate + phosphate
-
-
-
?
3-phosphonopyruvate + H2O
the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction
667335
Burkholderia cepacia
pyruvate + phosphate
-
-
-
?
3-phosphonopyruvate + H2O
-
667335
Burkholderia cepacia Pal6
pyruvate + phosphate
-
-
-
?
3-phosphonopyruvate + H2O
the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction
667335
Burkholderia cepacia Pal6
pyruvate + phosphate
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
-
Burkholderia cepacia
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Burkholderia cepacia
General Stability (protein specific)
General Stability
Organism
dialysis of cell-free extract for 24 h at 4C results in a 72% loss of activity
Burkholderia cepacia
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-phosphonopropionic acid
10 mM, 21% inhibition
Burkholderia cepacia
Aminomethylphosphonic acid
10 mM, 19% inhibition
Burkholderia cepacia
EDTA
Co2+, Ni2+, Mg2+, Zn2+, Fe3+ and Ca2+ recover activity. Cu2+, Mn2+ and Cs+ do not recover activity
Burkholderia cepacia
Fe2+
5 mM, 94% inhibition
Burkholderia cepacia
glyphosate
10 mM, 23% inhibition
Burkholderia cepacia
Mn2+
5 mM, 12% inhibition
Burkholderia cepacia
Phosphonoacetate
10 mM, 30% inhibition
Burkholderia cepacia
phosphonobutyric acid
10 mM, 14% inhibition
Burkholderia cepacia
Phosphonoformate
10 mM, 37% inhibition
Burkholderia cepacia
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.72
-
3-phosphonopyruvate
-
Burkholderia cepacia
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
5 mM, 1.5fold activation
Burkholderia cepacia
Co2+
5 mM, 4.8fold activation
Burkholderia cepacia
Cu2+
5 mM, 1.2fold activation
Burkholderia cepacia
Mg2+
5 mM, 3.4fold activation
Burkholderia cepacia
Ni2+
5 mM, 4.3fold activation
Burkholderia cepacia
Zn2+
5 mM, 1.8fold activation
Burkholderia cepacia
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
232000
-
non-denaturing PAGE
Burkholderia cepacia
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-phosphonopyruvate + H2O
Burkholderia cepacia
the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction
pyruvate + phosphate
-
-
?
3-phosphonopyruvate + H2O
Burkholderia cepacia Pal6
the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction
pyruvate + phosphate
-
-
?
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 10 weeks, 54% loss of activity
Burkholderia cepacia
20, 50% loss of activity after 3 days, complete loss of activity after 6 days
Burkholderia cepacia
4C, 30 days, 38% loss of activity
Burkholderia cepacia
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-phosphonopyruvate + H2O
-
667335
Burkholderia cepacia
pyruvate + phosphate
-
-
-
?
3-phosphonopyruvate + H2O
the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction
667335
Burkholderia cepacia
pyruvate + phosphate
-
-
-
?
3-phosphonopyruvate + H2O
-
667335
Burkholderia cepacia Pal6
pyruvate + phosphate
-
-
-
?
3-phosphonopyruvate + H2O
the activity is induced in presence of phosphonoalanine and does not require phosphate starvation for induction
667335
Burkholderia cepacia Pal6
pyruvate + phosphate
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
50
-
-
Burkholderia cepacia
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Burkholderia cepacia
Other publictions for EC 3.11.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
697989
Kulakova
Expression of the phosphonoala ...
Variovorax sp., Variovorax sp. Pal2
FEMS Microbiol. Lett.
292
100-106
2009
1
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667704
Chen
Structure and kinetics of phos ...
Variovorax sp., Variovorax sp. Pal2
Biochemistry
45
11491-11504
2006
-
-
1
1
1
-
4
1
-
3
2
-
-
5
-
-
1
-
-
-
-
-
4
1
-
-
-
2
-
-
-
-
4
-
-
-
-
1
-
1
1
-
-
4
4
1
-
3
2
-
-
-
-
1
-
-
-
-
4
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
669198
Kulakova
The purification and character ...
Variovorax sp., Variovorax sp. Pal2
J. Biol. Chem.
278
23426-23431
2003
-
-
1
-
-
-
6
1
-
3
2
2
-
5
-
-
1
-
-
-
1
1
4
1
-
-
1
-
1
-
-
-
-
1
-
-
-
1
-
-
-
-
-
6
-
1
-
3
2
2
-
-
-
1
-
-
1
1
4
1
-
-
1
-
1
-
-
1
-
-
-
-
-
-
667335
Ternan
Initial in vitro characterisat ...
Burkholderia cepacia, Burkholderia cepacia Pal6
Arch. Microbiol.
173
35-41
2000
-
-
-
-
-
1
9
1
-
6
1
2
-
3
-
-
-
-
-
-
-
3
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
9
-
1
-
6
1
2
-
-
-
-
-
-
-
3
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-