BRENDA - Enzyme Database
show all sequences of 3.11.1.2

Genetic and biochemical characterization of a pathway for the degradation of 2-aminoethylphosphonate in Sinorhizobium meliloti 1021

Borisova, S.A.; Christman, H.D.; Metcalf, M.E.; Zulkepli, N.A.; Zhang, J.K.; van der Donk, W.A.; Metcalf, W.W.; J. Biol. Chem. 286, 22283-22290 (2011)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene phnA, expression of N-terminally His6-tagged PhnA in Escherichia coli
Sinorhizobium meliloti
Engineering
Protein Variants
Commentary
Organism
additional information
construction of a phnA deletion mutant
Sinorhizobium meliloti
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetics analysis
Sinorhizobium meliloti
0.0221
-
Phosphonoacetate
pH 7.5, 30C, recombinant enzyme
Sinorhizobium meliloti
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
increases kcat and Km, decreasing kcat/KM, in the reaction
Sinorhizobium meliloti
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48400
-
x * 48400, recombinant His6-tagged PhnA, SDS-PAGE
Sinorhizobium meliloti
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
Phosphonoacetate + H2O
Sinorhizobium meliloti
-
acetate + phosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Sinorhizobium meliloti
-
gene phnA
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant N-terminally His6-tagged PhnA from Escherichia coli by nickel affinity chromatography
Sinorhizobium meliloti
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Phosphonoacetate + H2O
-
719938
Sinorhizobium meliloti
acetate + phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 48400, recombinant His6-tagged PhnA, SDS-PAGE
Sinorhizobium meliloti
Synonyms
Synonyms
Commentary
Organism
PhnA
-
Sinorhizobium meliloti
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Sinorhizobium meliloti
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.9
-
Phosphonoacetate
pH 7.5, 30C, recombinant enzyme
Sinorhizobium meliloti
1.1
-
Phosphonoacetate
pH 7.5, 30C, recombinant enzyme, with 0.01 mM Zn2+
Sinorhizobium meliloti
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Sinorhizobium meliloti
Cloned(Commentary) (protein specific)
Commentary
Organism
gene phnA, expression of N-terminally His6-tagged PhnA in Escherichia coli
Sinorhizobium meliloti
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
construction of a phnA deletion mutant
Sinorhizobium meliloti
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetics analysis
Sinorhizobium meliloti
0.0221
-
Phosphonoacetate
pH 7.5, 30C, recombinant enzyme
Sinorhizobium meliloti
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
increases kcat and Km, decreasing kcat/KM, in the reaction
Sinorhizobium meliloti
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
48400
-
x * 48400, recombinant His6-tagged PhnA, SDS-PAGE
Sinorhizobium meliloti
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
Phosphonoacetate + H2O
Sinorhizobium meliloti
-
acetate + phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant N-terminally His6-tagged PhnA from Escherichia coli by nickel affinity chromatography
Sinorhizobium meliloti
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Phosphonoacetate + H2O
-
719938
Sinorhizobium meliloti
acetate + phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 48400, recombinant His6-tagged PhnA, SDS-PAGE
Sinorhizobium meliloti
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Sinorhizobium meliloti
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.9
-
Phosphonoacetate
pH 7.5, 30C, recombinant enzyme
Sinorhizobium meliloti
1.1
-
Phosphonoacetate
pH 7.5, 30C, recombinant enzyme, with 0.01 mM Zn2+
Sinorhizobium meliloti
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Sinorhizobium meliloti
General Information
General Information
Commentary
Organism
metabolism
the enzyme catalyzes a step in the 2-aminoethylphosphonate degradation, conversion of 2-aminoethylphosphonate to phosphate and degradation of phosphonate compounds, pathway overview
Sinorhizobium meliloti
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme catalyzes a step in the 2-aminoethylphosphonate degradation, conversion of 2-aminoethylphosphonate to phosphate and degradation of phosphonate compounds, pathway overview
Sinorhizobium meliloti
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4100
-
Phosphonoacetate
pH 7.5, 30C, recombinant enzyme
Sinorhizobium meliloti
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4100
-
Phosphonoacetate
pH 7.5, 30C, recombinant enzyme
Sinorhizobium meliloti
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734593
Perry
A microfluidic approach for pr ...
Sinorhizobium meliloti
Lab Chip
13
3183-3187
2013
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1
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2
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1
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1
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1
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1
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-
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-
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-
-
-
-
733908
Villarreal-Chiu
The genes and enzymes of phosp ...
Pseudomonas fluorescens
Front. Microbiol.
3
19
2012
-
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1
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1
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2
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1
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1
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1
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1
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2
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2
2
-
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-
718897
Kim
Divergence of chemical functio ...
Pseudomonas fluorescens, Pseudomonas fluorescens 23F
Biochemistry
50
3481-3494
2011
1
-
1
1
-
-
3
-
-
3
2
2
-
6
-
-
1
1
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4
2
1
1
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1
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4
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1
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1
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1
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3
4
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3
2
2
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1
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4
2
1
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1
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2
2
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719273
Agarwal
Structural and mechanistic ins ...
Sinorhizobium meliloti
Chem. Biol.
18
1230-1240
2011
-
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1
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1
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1
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3
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1
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1
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1
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1
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1
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1
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-
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2
2
-
-
-
719938
Borisova
Genetic and biochemical charac ...
Sinorhizobium meliloti
J. Biol. Chem.
286
22283-22290
2011
-
-
1
-
1
-
-
2
-
1
1
1
-
4
-
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1
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-
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1
1
1
1
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2
1
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1
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1
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2
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1
1
1
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1
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1
1
1
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2
1
-
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1
1
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1
1
720399
Cooley
Phosphonoacetate biosynthesis: ...
Roseovarius nubinhibens
Microbiology
80
335-340
2011
-
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1
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3
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1
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1
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1
1
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-
-
697646
Gilbert
Potential for phosphonoacetate ...
Burkholderia ambifaria, Burkholderia ambifaria AMMD, Burkholderia multivorans, Candidatus Solibacter usitatus, Candidatus Solibacter usitatus Ellin6076, Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1, Emiliania huxleyi, Karenia mikimotoi, no activity in Vibrio shiloi, Paraburkholderia xenovorans, Photobacterium rosenbergii, Photobacterium rosenbergii CC006, Rhodopseudomonas palustris, Rhodopseudomonas palustris CGA009, Sinorhizobium medicae, Sinorhizobium medicae WSM419, Sinorhizobium meliloti, Sorangium cellulosum, Verminephrobacter eiseniae, Verminephrobacter eiseniae EF01-2, Vibrio campbellii, Vibrio campbellii CC028, Vibrio harveyi
Environ. Microbiol.
11
111-125
2009
-
-
15
-
-
-
-
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-
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28
-
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17
-
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670346
Forlani
Phosphonoacetic acid utilizati ...
Penicillium oxalicum
Mycol. Res.
110
1455-1463
2006
-
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-
-
-
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3
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1
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1
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683482
O'Loughlin
A role for carbon catabolite r ...
Agromyces fucosus, Agromyces fucosus Vs2
FEMS Microbiol. Lett.
261
133-140
2006
-
-
-
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4
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684053
Klimek-Ochab
Phosphonoacetate hydrolase fro ...
Penicillium oxalicum
Res. Microbiol.
157
125-135
2006
-
-
-
-
-
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1
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2
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3
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1
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1
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1
1
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1
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1
1
1
1
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1
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1
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2
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1
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1
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1
1
1
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1
1
1
1
1
-
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-
655816
McGrath
-
A comparison of three bacteria ...
Curtobacterium sp., Curtobacterium sp. PA1, Pseudomonas sp., Pseudomonas sp. PA2
J. Appl. Microbiol.
86
834-840
1999
-
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18
2
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8
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4
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4
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2
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8
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2
2
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2
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18
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2
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8
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4
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8
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2
2
2
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2
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-
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22933
Kulakova
Cloning of the phosphonoacetat ...
Pseudomonas fluorescens
Gene
195
49-53
1997
-
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1
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1
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4
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22930
McGrath
The purification and propertie ...
Pseudomonas fluorescens 23F, Pseudomonas fluorescens
Eur. J. Biochem.
234
225-230
1995
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1
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5
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5
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1
1
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22931
McGrath
-
A plate assay for the detectio ...
Pseudomonas fluorescens, Pseudomonas fluorescens 23F
Biotechnol. Tech.
9
497-502
1995
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22932
McMullan
In vitro characterization of a ...
Pseudomonas fluorescens
J. Bacteriol.
176
320-324
1994
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6
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6
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