BRENDA - Enzyme Database
show all sequences of 3.11.1.2

Phosphonoacetate hydrolase from Penicillium oxalicum: Purification and properties, phosphate starvation-independent expression, and partial sequencing

Klimek-Ochab, M.; Raucci, G.; Lejczak, B.; Forlani, G.; Res. Microbiol. 157, 125-135 (2006)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.9
-
Phosphonoacetate
pH 8.0, 35C
Penicillium oxalicum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42600
-
gel filtration
Penicillium oxalicum
43200
-
1 * 43200, SDS-PAGE
Penicillium oxalicum
Organism
Organism
UniProt
Commentary
Textmining
Penicillium oxalicum
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Penicillium oxalicum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
665
-
pH 8.0, 35C
Penicillium oxalicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Phosphonoacetate + H2O
-
684053
Penicillium oxalicum
acetate + phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 43200, SDS-PAGE
Penicillium oxalicum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
52
-
-
Penicillium oxalicum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.7
-
Phosphonoacetate
pH 8.0, 35C
Penicillium oxalicum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Penicillium oxalicum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
8.6
more than 90% of maximum activity
Penicillium oxalicum
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.2
-
above pH 6.2 stable for at least 10 min
Penicillium oxalicum
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Penicillium oxalicum
isoelectric focusing
-
7.1
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.9
-
Phosphonoacetate
pH 8.0, 35C
Penicillium oxalicum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42600
-
gel filtration
Penicillium oxalicum
43200
-
1 * 43200, SDS-PAGE
Penicillium oxalicum
Purification (Commentary) (protein specific)
Commentary
Organism
-
Penicillium oxalicum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
665
-
pH 8.0, 35C
Penicillium oxalicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Phosphonoacetate + H2O
-
684053
Penicillium oxalicum
acetate + phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 43200, SDS-PAGE
Penicillium oxalicum
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
52
-
-
Penicillium oxalicum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.7
-
Phosphonoacetate
pH 8.0, 35C
Penicillium oxalicum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Penicillium oxalicum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
8.6
more than 90% of maximum activity
Penicillium oxalicum
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.2
-
above pH 6.2 stable for at least 10 min
Penicillium oxalicum
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Penicillium oxalicum
isoelectric focusing
-
7.1
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734593
Perry
A microfluidic approach for pr ...
Sinorhizobium meliloti
Lab Chip
13
3183-3187
2013
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1
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733908
Villarreal-Chiu
The genes and enzymes of phosp ...
Pseudomonas fluorescens
Front. Microbiol.
3
19
2012
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1
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2
2
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718897
Kim
Divergence of chemical functio ...
Pseudomonas fluorescens, Pseudomonas fluorescens 23F
Biochemistry
50
3481-3494
2011
1
-
1
1
-
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3
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3
2
2
-
6
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1
1
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4
2
1
1
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1
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4
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1
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1
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1
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3
4
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3
2
2
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1
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4
2
1
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1
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2
2
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719273
Agarwal
Structural and mechanistic ins ...
Sinorhizobium meliloti
Chem. Biol.
18
1230-1240
2011
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1
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3
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1
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2
2
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719938
Borisova
Genetic and biochemical charac ...
Sinorhizobium meliloti
J. Biol. Chem.
286
22283-22290
2011
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1
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1
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2
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1
1
1
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4
-
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1
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1
1
1
1
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1
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1
1
1
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1
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1
1
1
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2
1
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1
1
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1
1
720399
Cooley
Phosphonoacetate biosynthesis: ...
Roseovarius nubinhibens
Microbiology
80
335-340
2011
-
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1
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3
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1
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1
1
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697646
Gilbert
Potential for phosphonoacetate ...
Burkholderia ambifaria, Burkholderia ambifaria AMMD, Burkholderia multivorans, Candidatus Solibacter usitatus, Candidatus Solibacter usitatus Ellin6076, Cupriavidus necator, Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1, Emiliania huxleyi, Karenia mikimotoi, no activity in Vibrio shiloi, Paraburkholderia xenovorans, Photobacterium rosenbergii, Photobacterium rosenbergii CC006, Rhodopseudomonas palustris, Rhodopseudomonas palustris CGA009, Sinorhizobium medicae, Sinorhizobium medicae WSM419, Sinorhizobium meliloti, Sorangium cellulosum, Verminephrobacter eiseniae, Verminephrobacter eiseniae EF01-2, Vibrio campbellii, Vibrio campbellii CC028, Vibrio harveyi
Environ. Microbiol.
11
111-125
2009
-
-
15
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28
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17
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670346
Forlani
Phosphonoacetic acid utilizati ...
Penicillium oxalicum
Mycol. Res.
110
1455-1463
2006
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683482
O'Loughlin
A role for carbon catabolite r ...
Agromyces fucosus, Agromyces fucosus Vs2
FEMS Microbiol. Lett.
261
133-140
2006
-
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684053
Klimek-Ochab
Phosphonoacetate hydrolase fro ...
Penicillium oxalicum
Res. Microbiol.
157
125-135
2006
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3
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1
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1
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2
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1
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1
1
1
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1
1
1
1
1
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655816
McGrath
-
A comparison of three bacteria ...
Curtobacterium sp., Curtobacterium sp. PA1, Pseudomonas sp., Pseudomonas sp. PA2
J. Appl. Microbiol.
86
834-840
1999
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2
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8
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4
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4
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2
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8
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2
2
2
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2
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18
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2
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8
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4
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2
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8
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2
2
2
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2
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22933
Kulakova
Cloning of the phosphonoacetat ...
Pseudomonas fluorescens
Gene
195
49-53
1997
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1
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1
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4
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22930
McGrath
The purification and propertie ...
Pseudomonas fluorescens 23F, Pseudomonas fluorescens
Eur. J. Biochem.
234
225-230
1995
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1
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5
1
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3
2
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5
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1
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1
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1
2
1
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1
1
1
1
1
1
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1
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5
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1
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3
2
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1
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1
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1
2
1
1
1
1
1
1
1
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22931
McGrath
-
A plate assay for the detectio ...
Pseudomonas fluorescens, Pseudomonas fluorescens 23F
Biotechnol. Tech.
9
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1995
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4
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22932
McMullan
In vitro characterization of a ...
Pseudomonas fluorescens
J. Bacteriol.
176
320-324
1994
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6
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1
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3
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