Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 34% inhibition at 1 mM, 76% inhibition at 10 mM | Curtobacterium sp. | |
1,10-phenanthroline | complete inhibition at 1 mM | Pseudomonas sp. | |
2-mercaptoethanol | 73% inhibition at 10 mM | Curtobacterium sp. | |
2-mercaptoethanol | 58% inhibition at 10 mM | Pseudomonas sp. | |
2-phosphonopropionic acid | 33% inhibition at 10 mM | Curtobacterium sp. | |
2-phosphonopropionic acid | about 30% inhibition at 10 mM | Pseudomonas sp. | |
3-phosphonopropionic acid | about 30% inhibition at 10 mM | Pseudomonas sp. | |
dithiothreitol | 47% inhibition at 10 mM | Curtobacterium sp. | |
dithiothreitol | 47% inhibition at 10 mM | Pseudomonas sp. | |
EDTA | 50% inhibition at 2 mM | Curtobacterium sp. | |
EDTA | complete inhibition at 2 mM | Pseudomonas sp. | |
iminodiacetic acid | 64% inhibition at 10 mM | Curtobacterium sp. | |
iminodiacetic acid | 64% inhibition at 10 mM | Pseudomonas sp. | |
Mg2+ | slight inhibition of 17% at 1 mM | Pseudomonas sp. | |
additional information | reaction is phosphate insensitive, no inhibition by iodoacetic acid, chloroacetic acid, acetic acid, phosphorous acid | Curtobacterium sp. | |
additional information | reaction is phosphate insensitive, no inhibition by iodoacetic acid, chloroacetic acid, acetic acid, phosphorous acid | Pseudomonas sp. | |
Phosphonoformic acid | about 70% inhibition at 10 mM | Curtobacterium sp. | |
Phosphonoformic acid | about 70% inhibition at 10 mM | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0078 | - |
Phosphonoacetate | pH 7.5, 30°C | Curtobacterium sp. | |
1.5 | - |
Phosphonoacetate | pH 7.5, 30°C, 1 mM Zn2+ | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates 3.75fold at 1 mM | Pseudomonas sp. | |
Co2+ | slightly activates 1.1fold at 1 mM | Curtobacterium sp. | |
Mg2+ | slightly activates 1.1fold at 1 mM | Curtobacterium sp. | |
Mn2+ | activates 2.7fold at 1 mM | Pseudomonas sp. | |
additional information | reaction is phosphate insensitive, no effect by Fe3+ or Ca2+, no activation by organophosphononate analogues | Pseudomonas sp. | |
additional information | reaction is phosphate insensitive, no effect by Mn2+ or Fe3+, no activation by organophosphononate analogues | Curtobacterium sp. | |
Zn2+ | 1.3fold activation at 1 mM | Curtobacterium sp. | |
Zn2+ | dependent on, 4-7fold activation at 1 mM | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Phosphonoacetate + H2O | Pseudomonas sp. | route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM | acetate + phosphate | - |
? | |
Phosphonoacetate + H2O | Curtobacterium sp. | route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM | acetate + phosphate | - |
? | |
Phosphonoacetate + H2O | Pseudomonas sp. PA2 | route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM | acetate + phosphate | - |
? | |
Phosphonoacetate + H2O | Curtobacterium sp. PA1 | route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM | acetate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Curtobacterium sp. | - |
substrate-inducible enzyme, expression is independent of the phosphate status of the cell | - |
Curtobacterium sp. PA1 | - |
substrate-inducible enzyme, expression is independent of the phosphate status of the cell | - |
Pseudomonas sp. | - |
substrate-inducible enzyme, expression is independent of the phosphate status of the cell | - |
Pseudomonas sp. PA2 | - |
substrate-inducible enzyme, expression is independent of the phosphate status of the cell | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:phosphonoacetate-grown cell | linear yield of enzyme in 0-100 mM phosphonoacetate, decrease in activity at higher concentrations | Pseudomonas sp. | - |
culture condition:phosphonoacetate-grown cell | linear yield of enzyme in 0-40 mM phosphonoacetate, decrease in activity at higher concentrations | Curtobacterium sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Phosphonoacetate + H2O | route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM | Pseudomonas sp. | acetate + phosphate | - |
? | |
Phosphonoacetate + H2O | route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM | Curtobacterium sp. | acetate + phosphate | - |
? | |
Phosphonoacetate + H2O | absolutely specific for, overview | Pseudomonas sp. | acetate + phosphate | - |
? | |
Phosphonoacetate + H2O | absolutely specific for, overview | Curtobacterium sp. | acetate + phosphate | - |
? | |
Phosphonoacetate + H2O | route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM | Pseudomonas sp. PA2 | acetate + phosphate | - |
? | |
Phosphonoacetate + H2O | absolutely specific for, overview | Pseudomonas sp. PA2 | acetate + phosphate | - |
? | |
Phosphonoacetate + H2O | route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM | Curtobacterium sp. PA1 | acetate + phosphate | - |
? | |
Phosphonoacetate + H2O | absolutely specific for, overview | Curtobacterium sp. PA1 | acetate + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Pseudomonas sp. |
60 | - |
- |
Curtobacterium sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 75 | half-maximal activity at 40 and 75°C | Pseudomonas sp. |
40 | 75 | half-maximal activity at 40 and 75°C | Curtobacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, 70% remaining activity, rapid inactivation above | Curtobacterium sp. |
60 | - |
30 min, 78% remaining activity, rapid inactivation above | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas sp. |
8 | - |
- |
Curtobacterium sp. |