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Literature summary for 3.11.1.2 extracted from

  • McGrath, J.W.; Kulakova, A.N.; Quinn, J.P.
    A comparison of three bacterial phosphonoacetate hydrolases from different environmental sources (1999), J. Appl. Microbiol., 86, 834-840.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 34% inhibition at 1 mM, 76% inhibition at 10 mM Curtobacterium sp.
1,10-phenanthroline complete inhibition at 1 mM Pseudomonas sp.
2-mercaptoethanol 73% inhibition at 10 mM Curtobacterium sp.
2-mercaptoethanol 58% inhibition at 10 mM Pseudomonas sp.
2-phosphonopropionic acid 33% inhibition at 10 mM Curtobacterium sp.
2-phosphonopropionic acid about 30% inhibition at 10 mM Pseudomonas sp.
3-phosphonopropionic acid about 30% inhibition at 10 mM Pseudomonas sp.
dithiothreitol 47% inhibition at 10 mM Curtobacterium sp.
dithiothreitol 47% inhibition at 10 mM Pseudomonas sp.
EDTA 50% inhibition at 2 mM Curtobacterium sp.
EDTA complete inhibition at 2 mM Pseudomonas sp.
iminodiacetic acid 64% inhibition at 10 mM Curtobacterium sp.
iminodiacetic acid 64% inhibition at 10 mM Pseudomonas sp.
Mg2+ slight inhibition of 17% at 1 mM Pseudomonas sp.
additional information reaction is phosphate insensitive, no inhibition by iodoacetic acid, chloroacetic acid, acetic acid, phosphorous acid Curtobacterium sp.
additional information reaction is phosphate insensitive, no inhibition by iodoacetic acid, chloroacetic acid, acetic acid, phosphorous acid Pseudomonas sp.
Phosphonoformic acid about 70% inhibition at 10 mM Curtobacterium sp.
Phosphonoformic acid about 70% inhibition at 10 mM Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0078
-
Phosphonoacetate pH 7.5, 30°C Curtobacterium sp.
1.5
-
Phosphonoacetate pH 7.5, 30°C, 1 mM Zn2+ Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates 3.75fold at 1 mM Pseudomonas sp.
Co2+ slightly activates 1.1fold at 1 mM Curtobacterium sp.
Mg2+ slightly activates 1.1fold at 1 mM Curtobacterium sp.
Mn2+ activates 2.7fold at 1 mM Pseudomonas sp.
additional information reaction is phosphate insensitive, no effect by Fe3+ or Ca2+, no activation by organophosphononate analogues Pseudomonas sp.
additional information reaction is phosphate insensitive, no effect by Mn2+ or Fe3+, no activation by organophosphononate analogues Curtobacterium sp.
Zn2+ 1.3fold activation at 1 mM Curtobacterium sp.
Zn2+ dependent on, 4-7fold activation at 1 mM Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Phosphonoacetate + H2O Pseudomonas sp. route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM acetate + phosphate
-
?
Phosphonoacetate + H2O Curtobacterium sp. route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM acetate + phosphate
-
?
Phosphonoacetate + H2O Pseudomonas sp. PA2 route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM acetate + phosphate
-
?
Phosphonoacetate + H2O Curtobacterium sp. PA1 route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM acetate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Curtobacterium sp.
-
substrate-inducible enzyme, expression is independent of the phosphate status of the cell
-
Curtobacterium sp. PA1
-
substrate-inducible enzyme, expression is independent of the phosphate status of the cell
-
Pseudomonas sp.
-
substrate-inducible enzyme, expression is independent of the phosphate status of the cell
-
Pseudomonas sp. PA2
-
substrate-inducible enzyme, expression is independent of the phosphate status of the cell
-

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:phosphonoacetate-grown cell linear yield of enzyme in 0-100 mM phosphonoacetate, decrease in activity at higher concentrations Pseudomonas sp.
-
culture condition:phosphonoacetate-grown cell linear yield of enzyme in 0-40 mM phosphonoacetate, decrease in activity at higher concentrations Curtobacterium sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Phosphonoacetate + H2O route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM Pseudomonas sp. acetate + phosphate
-
?
Phosphonoacetate + H2O route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM Curtobacterium sp. acetate + phosphate
-
?
Phosphonoacetate + H2O absolutely specific for, overview Pseudomonas sp. acetate + phosphate
-
?
Phosphonoacetate + H2O absolutely specific for, overview Curtobacterium sp. acetate + phosphate
-
?
Phosphonoacetate + H2O route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM Pseudomonas sp. PA2 acetate + phosphate
-
?
Phosphonoacetate + H2O absolutely specific for, overview Pseudomonas sp. PA2 acetate + phosphate
-
?
Phosphonoacetate + H2O route of microbial metabolism of xenobiotic organophosphonates, substrate can serve as the sole source of carbon, energy and phosphate for the organism in the range of 40-100 mM Curtobacterium sp. PA1 acetate + phosphate
-
?
Phosphonoacetate + H2O absolutely specific for, overview Curtobacterium sp. PA1 acetate + phosphate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Pseudomonas sp.
60
-
-
Curtobacterium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 75 half-maximal activity at 40 and 75°C Pseudomonas sp.
40 75 half-maximal activity at 40 and 75°C Curtobacterium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
30 min, 70% remaining activity, rapid inactivation above Curtobacterium sp.
60
-
30 min, 78% remaining activity, rapid inactivation above Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas sp.
8
-
-
Curtobacterium sp.