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Literature summary for 3.11.1.1 extracted from

  • Olsen, D.B.; Hepburn, T.W.; Lee, S.l.; Martin, B.M.; Mariano, P.S.; Dunaway-Mariano, D.
    Investigation of the substrate binding and catalytic groups of the P-C bond cleaving enzyme, phosphonoacetaldehyde hydrolase (1992), Arch. Biochem. Biophys., 296, 144-151.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
acetonylphosphonate in presence of NH4+ Bacillus cereus
fluorophosphate competitive inhibitor Bacillus cereus
malonic semialdehyde competitive inhibitor Bacillus cereus
phosphonoacetaldehyde competitive inhibitor Bacillus cereus
phosphonoethanol competitive inhibitor Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
AI-2
-
Bacillus cereus AI-2
-
AI-2
-

Reaction

Reaction Comment Organism Reaction ID
phosphonoacetaldehyde + H2O = acetaldehyde + phosphate Schiff base formation with catalytic Lys and phosphonoacetaldehyde, PC-bond cleavage in the Schiff base takes place during the second partial reaction and liberation of the acetaldehyde from the resulting enamine occurs during the third partial reaction Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetonyl phosphonate + H2O
-
Bacillus cereus ?
-
?
acetonyl phosphonate + H2O
-
Bacillus cereus AI-2 ?
-
?
phosphonoacetylaldehyde + H2O
-
Bacillus cereus acetaldehyde + phosphate
-
?
phosphonoacetylaldehyde + H2O
-
Bacillus cereus AI-2 acetaldehyde + phosphate
-
?
thiophosphonoacetaldehyde + H2O
-
Bacillus cereus thiophosphate + acetaldehyde
-
?
thiophosphonoacetaldehyde + H2O
-
Bacillus cereus AI-2 thiophosphate + acetaldehyde
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 8
-
Bacillus cereus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
protonation of the active site Lys is the cause for loss of activity Bacillus cereus
8
-
deprotonation of the active site Cys may be the cause for the loss of enzyme activity Bacillus cereus