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Literature summary for 3.10.1.2 extracted from

  • Niimura, T.; Tokiedo, T.; Yamaha, T.
    Partial purification and some properties of cyclamate sulfamatase (1974), J. Biochem., 75, 407-417.
    View publication on PubMed

General Stability

General Stability Organism
gelatin is effective stabilizer Pseudomonas sp.
in Tris buffer minor loss of activity than in phosphate buffer Pseudomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
BaCl2
-
Pseudomonas sp.
Cd2+
-
Pseudomonas sp.
CoSO4
-
Pseudomonas sp.
CuSO4
-
Pseudomonas sp.
Cyclohexylamine
-
Pseudomonas sp.
EDTA metal ions reverse inhibition Pseudomonas sp.
H3BO3
-
Pseudomonas sp.
Hg2+
-
Pseudomonas sp.
iodoacetic acid
-
Pseudomonas sp.
MgSO4
-
Pseudomonas sp.
MnCl2
-
Pseudomonas sp.
Na2B4O7
-
Pseudomonas sp.
Na2CO3
-
Pseudomonas sp.
Na2SO3
-
Pseudomonas sp.
NaN3
-
Pseudomonas sp.
NH2OH
-
Pseudomonas sp.
(NH4)2SO4
-
Pseudomonas sp.
NiSO4
-
Pseudomonas sp.
p-chloromercuribenzoate
-
Pseudomonas sp.
phosphate
-
Pseudomonas sp.
potassium citrate
-
Pseudomonas sp.
ZnSO4
-
Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5
-
cyclamate
-
Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulation Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. K
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
160
-
-
Pseudomonas sp.

Storage Stability

Storage Stability Organism
-20°C, 0.04 M Tris-HCl buffer, pH 7.2, 0.2% gelatin, 3 months, 10% loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-cyclohexylsulfamate + H2O
-
Pseudomonas sp. cyclohexylamine + sulfate
-
?
N-cyclohexylsulfamate + H2O
-
Pseudomonas sp. K cyclohexylamine + sulfate
-
?
N-(n-propyl)sulfamate + H2O
-
Pseudomonas sp. n-propylamine + sulfate
-
?
N-(n-propyl)sulfamate + H2O
-
Pseudomonas sp. K n-propylamine + sulfate
-
?
N-(n-amyl)sulfamate + H2O
-
Pseudomonas sp. n-amylamine + sulfate
-
?
N-(n-amyl)sulfamate + H2O
-
Pseudomonas sp. K n-amylamine + sulfate
-
?
N-(n-hexyl)sulfamate + H2O
-
Pseudomonas sp. n-hexylamine + sulfate
-
?
N-(n-hexyl)sulfamate + H2O
-
Pseudomonas sp. K n-hexylamine + sulfate
-
?
N-(n-octyl)sulfamate + H2O
-
Pseudomonas sp. n-octylamine + sulfate
-
?
N-(n-octyl)sulfamate + H2O
-
Pseudomonas sp. K n-octylamine + sulfate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
2 h without loss of activity Pseudomonas sp.
70
-
completely inactivated after 5 min Pseudomonas sp.
60
-
40% loss of activity after 20 min Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 6.7
-
Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.9 7.5
-
Pseudomonas sp.