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Literature summary for 3.10.1.1 extracted from
- Flavobacterium heparinum sulphamidase for D-glucosamine sulphamate. Purification and characterisation (1987), Eur. J. Biochem., 165, 633-638.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| PO43- | 5 mM decreases activity to 71% | Pedobacter heparinus |  |
| SO42- | 5 mM decreases activity to 72% | Pedobacter heparinus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0083 | - |
2-deoxy-2-sulfamido-D-glucose | purified enzyme | Pedobacter heparinus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Pedobacter heparinus | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM essential for activity | Pedobacter heparinus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 81500 | - |
SDS-PAGE | Pedobacter heparinus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pedobacter heparinus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pedobacter heparinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-2-sulfamido-D-glucose + H2O | - |
Pedobacter heparinus | 2-deoxy-2-amino-D-glucose + sulfate | - |
ir | |
| glucosamine 2,6-disulfate + H2O | no activity with purified enzyme | Pedobacter heparinus | D-glucosamine 6-sulfate + sulfate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pedobacter heparinus |
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