Any feedback?
Literature summary for 3.1.99.B1 extracted from
- Serial intermediates with a 1 nt 3'-flap and 5' variable-length flaps are formed by cooperative functioning of Pyrococcus horikoshii FEN-1 with either B or D DNA polymerases (2014), Extremophiles, 18, 415-427 .
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | O50123 | - |
- |
| Pyrococcus horikoshii OT-3 | O50123 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | FEN-1 has both 5'-flap endonuclease and 5'-3' exonuclease activities, FEN-1 activity is elevated by the presence of a 1 nucleotide expansion at the 3' end in the upstream primer of substrates called: structures with a 1 nt 3'-flap, which appear to be the most preferable substrates for FEN-1. Serial intermediates with a 1 nt 3'-flap and 5' variable-length flaps are formed by cooperative functioning of Pyrococcus horikoshii FEN-1 with either B or D DNA polymerases | Pyrococcus horikoshii | ? | - |
? |  |
| additional information | FEN-1 has both 5'-flap endonuclease and 5'-3' exonuclease activities, FEN-1 activity is elevated by the presence of a 1 nucleotide expansion at the 3' end in the upstream primer of substrates called: structures with a 1 nt 3'-flap, which appear to be the most preferable substrates for FEN-1. Serial intermediates with a 1 nt 3'-flap and 5' variable-length flaps are formed by cooperative functioning of Pyrococcus horikoshii FEN-1 with either B or D DNA polymerases | Pyrococcus horikoshii OT-3 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FEN-1 | - |
Pyrococcus horikoshii |
| flap endonuclease-1 | - |
Pyrococcus horikoshii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Pyrococcus horikoshii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Pyrococcus horikoshii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | plays important roles with DNA polymerases in DNA replication, repair and recombination | Pyrococcus horikoshii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
