Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activation, less effective than DTT | Pseudoalteromonas undina | |
DTT | activation | Pseudoalteromonas undina |
General Stability | Organism |
---|---|
2-mercaptoethanol enhances stability | Pseudoalteromonas undina |
DTT enhances stability | Pseudoalteromonas undina |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EGTA | Mn2+ reverses and stimulates over pre-EGTA-treatment activity level, Co2+ partially restores; reversible by Mn2+ or Co2+; strong | Pseudoalteromonas undina | |
Fe2+ | weak | Pseudoalteromonas undina | |
iodoacetic acid | - |
Pseudoalteromonas undina | |
N-ethylmaleimide | - |
Pseudoalteromonas undina | |
Ni2+ | weak | Pseudoalteromonas undina | |
Zn2+ | - |
Pseudoalteromonas undina |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activation | Pseudoalteromonas undina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
1 * 53000 SDS-PAGE | Pseudoalteromonas undina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas undina | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudoalteromonas undina |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
327.8 | - |
- |
Pseudoalteromonas undina |
575 | - |
- |
Pseudoalteromonas undina |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-ethyl(phenyl)phosphinate + H2O | i.e. 4-nitrophenylethyl phenylphosphinate | Pseudoalteromonas undina | ? | - |
? | |
4-nitrophenyl-ethyl(phenyl)phosphinate + H2O | at 46% the rate of diisopropyl fluorophosphate hydrolysis | Pseudoalteromonas undina | ? | - |
? | |
4-nitrophenyl-methyl(phenyl)phosphinate + H2O | i.e. 4-nitrophenylmethyl phenylphosphinate | Pseudoalteromonas undina | ? | - |
? | |
4-nitrophenyl-methyl(phenyl)phosphinate + H2O | at 28% the rate of diisopropyl fluorophosphate hydrolysis | Pseudoalteromonas undina | ? | - |
? | |
4-nitrophenylisopropyl phenylphosphinate + H2O | at 22% the rate of diisopropyl fluorophosphate hydrolysis | Pseudoalteromonas undina | ? | - |
? | |
4-nitrophenylpropyl phenylphosphinate + H2O | at 24% the rate of diisopropyl fluorophosphate hydrolysis | Pseudoalteromonas undina | ? | - |
? | |
diisopropyl fluorophosphate + H2O | at about 30% of O-pinacolylmethylphosphonofluoridate hydrolysis | Pseudoalteromonas undina | diisopropyl phosphate + fluoride | - |
? | |
diisopropyl fluorophosphate + H2O | i.e. DFP | Pseudoalteromonas undina | diisopropyl phosphate + fluoride | - |
? | |
mipafox + H2O | i.e. N,N'-diisopropyl phosphorodiamidofluoridate, poor substrate | Pseudoalteromonas undina | ? | - |
? | |
additional information | not: 4-nitrophenylphosphate | Pseudoalteromonas undina | ? | - |
? | |
additional information | 4-nitrophenylacetate, bis(4-nitrophenyl)phosphate, tris(4-nitrophenyl)phosphate | Pseudoalteromonas undina | ? | - |
? | |
O-cyclohexylmethylphosphonofluoridate + H2O | at 114% the rate of diisopropyl fluorophosphate hydrolysis | Pseudoalteromonas undina | ? | - |
? | |
O-pinacolylmethylphosphonofluoridate + H2O | best substrate | Pseudoalteromonas undina | ? | - |
? | |
paraoxon + H2O | i.e. diethyl-4-nitrophenyl phosphate, poor substrate | Pseudoalteromonas undina | diethylphosphate + 4-nitrophenol | - |
? | |
sarin + H2O | at 38% the rate of diisopropyl fluorophosphate hydrolysis | Pseudoalteromonas undina | ? | - |
? | |
tabun + H2O | i.e. N,N-dimethylethylphosphoramidocyanidate, at 124% the rate of diisopropyl fluorophosphate hydrolysis | Pseudoalteromonas undina | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 53000 SDS-PAGE | Pseudoalteromonas undina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudoalteromonas undina |
55 | - |
- |
Pseudoalteromonas undina |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
917 | - |
diisopropyl fluorophosphate | value above | Pseudoalteromonas undina |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudoalteromonas undina |