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Literature summary for 3.1.8.2 extracted from
- Purification and properties of a highly active organophosphorus acid anhydrolase from Alteromonas undina (1993), Appl. Environ. Microbiol., 59, 3138-3140.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | activation, less effective than DTT | Pseudoalteromonas undina |  |
| DTT | activation | Pseudoalteromonas undina | |
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol enhances stability | Pseudoalteromonas undina |
| DTT enhances stability | Pseudoalteromonas undina |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EGTA | Mn2+ reverses and stimulates over pre-EGTA-treatment activity level, Co2+ partially restores; reversible by Mn2+ or Co2+; strong | Pseudoalteromonas undina | |
| Fe2+ | weak | Pseudoalteromonas undina | |
| iodoacetic acid | - |
Pseudoalteromonas undina | |
| N-ethylmaleimide | - |
Pseudoalteromonas undina | |
| Ni2+ | weak | Pseudoalteromonas undina | |
| Zn2+ | - |
Pseudoalteromonas undina | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activation | Pseudoalteromonas undina | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
1 * 53000 SDS-PAGE | Pseudoalteromonas undina |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudoalteromonas undina | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudoalteromonas undina |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 327.8 | - |
- |
Pseudoalteromonas undina |
| 575 | - |
- |
Pseudoalteromonas undina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl-ethyl(phenyl)phosphinate + H2O | i.e. 4-nitrophenylethyl phenylphosphinate | Pseudoalteromonas undina | ? | - |
? | |
| 4-nitrophenyl-ethyl(phenyl)phosphinate + H2O | at 46% the rate of diisopropyl fluorophosphate hydrolysis | Pseudoalteromonas undina | ? | - |
? | |
| 4-nitrophenyl-methyl(phenyl)phosphinate + H2O | i.e. 4-nitrophenylmethyl phenylphosphinate | Pseudoalteromonas undina | ? | - |
? | |
| 4-nitrophenyl-methyl(phenyl)phosphinate + H2O | at 28% the rate of diisopropyl fluorophosphate hydrolysis | Pseudoalteromonas undina | ? | - |
? | |
| 4-nitrophenylisopropyl phenylphosphinate + H2O | at 22% the rate of diisopropyl fluorophosphate hydrolysis | Pseudoalteromonas undina | ? | - |
? | |
| 4-nitrophenylpropyl phenylphosphinate + H2O | at 24% the rate of diisopropyl fluorophosphate hydrolysis | Pseudoalteromonas undina | ? | - |
? | |
| diisopropyl fluorophosphate + H2O | at about 30% of O-pinacolylmethylphosphonofluoridate hydrolysis | Pseudoalteromonas undina | diisopropyl phosphate + fluoride | - |
? | |
| diisopropyl fluorophosphate + H2O | i.e. DFP | Pseudoalteromonas undina | diisopropyl phosphate + fluoride | - |
? | |
| mipafox + H2O | i.e. N,N'-diisopropyl phosphorodiamidofluoridate, poor substrate | Pseudoalteromonas undina | ? | - |
? | |
| additional information | not: 4-nitrophenylphosphate | Pseudoalteromonas undina | ? | - |
? | |
| additional information | 4-nitrophenylacetate, bis(4-nitrophenyl)phosphate, tris(4-nitrophenyl)phosphate | Pseudoalteromonas undina | ? | - |
? | |
| O-cyclohexylmethylphosphonofluoridate + H2O | at 114% the rate of diisopropyl fluorophosphate hydrolysis | Pseudoalteromonas undina | ? | - |
? | |
| O-pinacolylmethylphosphonofluoridate + H2O | best substrate | Pseudoalteromonas undina | ? | - |
? | |
| paraoxon + H2O | i.e. diethyl-4-nitrophenyl phosphate, poor substrate | Pseudoalteromonas undina | diethylphosphate + 4-nitrophenol | - |
? | |
| sarin + H2O | at 38% the rate of diisopropyl fluorophosphate hydrolysis | Pseudoalteromonas undina | ? | - |
? | |
| tabun + H2O | i.e. N,N-dimethylethylphosphoramidocyanidate, at 124% the rate of diisopropyl fluorophosphate hydrolysis | Pseudoalteromonas undina | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 53000 SDS-PAGE | Pseudoalteromonas undina |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Pseudoalteromonas undina |
| 55 | - |
- |
Pseudoalteromonas undina |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 917 | - |
diisopropyl fluorophosphate | value above | Pseudoalteromonas undina | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pseudoalteromonas undina |
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