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Literature summary for 3.1.8.2 extracted from

  • DeFrank, J.J.; Cheng, T.C.
    Purification and properties of an organophosphorus acid anhydrase from a halophilic bacterial isolate (1991), J. Bacteriol., 173, 1938-1943.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation, less effective than DTT Alteromonas sp.
DTT activation Alteromonas sp.

General Stability

General Stability Organism
DTT enhances stability Alteromonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Alteromonas sp.
EGTA only slightly by Fe3+ or Ni2+, not by Cs2+, Cu2+, Mg2+, Ca2+ or Zn2+; reversible by Mn2+ or Co2+ Alteromonas sp.
iodoacetamide
-
Alteromonas sp.
Mipafox reversible Alteromonas sp.
N-ethylmaleimide
-
Alteromonas sp.
PCMB
-
Alteromonas sp.
Zn2+
-
Alteromonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Alteromonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activation Alteromonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
1 * 60000, SDS-PAGE, under reducing and nonreducing conditions Alteromonas sp.

Organism

Organism UniProt Comment Textmining
Alteromonas sp.
-
JD6.5, halophilic bacterium
-

Purification (Commentary)

Purification (Comment) Organism
-
Alteromonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
268
-
-
Alteromonas sp.

Storage Stability

Storage Stability Organism
-70°C, in the presence of DTT, several months Alteromonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-ethyl(phenyl)phosphinate + H2O 40% the rate of diisopropyl fluorophosphate hydrolysis Alteromonas sp. ?
-
?
4-nitrophenyl-methyl(phenyl)phosphinate + H2O at 49% the rate of diisopropyl fluorophosphate hydrolysis Alteromonas sp. ?
-
?
diisopropyl fluorophosphate + H2O i.e. DFP Alteromonas sp. diisopropyl phosphate + fluoride
-
?
additional information not: 4-nitrophenylphosphonate, 4-nitrophenylphosphinorylcholine Alteromonas sp. ?
-
?
additional information not: 4-nitrophenylphosphate Alteromonas sp. ?
-
?
additional information 4-nitrophenylacetate, bis(4-nitrophenyl)phosphate, tris(4-nitrophenyl)phosphate Alteromonas sp. ?
-
?
sarin + H2O at 20% the rate of diisopropyl fluorophosphate hydrolysis Alteromonas sp. ?
-
?
soman + H2O at 240% the rate of diisopropyl fluorophosphate hydrolysis Alteromonas sp. ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE, under reducing and nonreducing conditions Alteromonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
3 mM diisopropyl fluorophosphate, pH 7.2, 1 mM MnCl2 Alteromonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
583
-
diisopropyl fluorophosphate pH 7.5, 22°C Alteromonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
diisopropyl fluorophosphate Alteromonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
7.6 9 about half-maximal activity at pH 7.6 and about 60% of maximal activity at pH 9 Alteromonas sp.