Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activation, less effective than DTT | Alteromonas sp. | |
DTT | activation | Alteromonas sp. |
General Stability | Organism |
---|---|
DTT enhances stability | Alteromonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Alteromonas sp. | |
EGTA | only slightly by Fe3+ or Ni2+, not by Cs2+, Cu2+, Mg2+, Ca2+ or Zn2+; reversible by Mn2+ or Co2+ | Alteromonas sp. | |
iodoacetamide | - |
Alteromonas sp. | |
Mipafox | reversible | Alteromonas sp. | |
N-ethylmaleimide | - |
Alteromonas sp. | |
PCMB | - |
Alteromonas sp. | |
Zn2+ | - |
Alteromonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Alteromonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activation | Alteromonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
1 * 60000, SDS-PAGE, under reducing and nonreducing conditions | Alteromonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alteromonas sp. | - |
JD6.5, halophilic bacterium | - |
Purification (Comment) | Organism |
---|---|
- |
Alteromonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
268 | - |
- |
Alteromonas sp. |
Storage Stability | Organism |
---|---|
-70°C, in the presence of DTT, several months | Alteromonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-ethyl(phenyl)phosphinate + H2O | 40% the rate of diisopropyl fluorophosphate hydrolysis | Alteromonas sp. | ? | - |
? | |
4-nitrophenyl-methyl(phenyl)phosphinate + H2O | at 49% the rate of diisopropyl fluorophosphate hydrolysis | Alteromonas sp. | ? | - |
? | |
diisopropyl fluorophosphate + H2O | i.e. DFP | Alteromonas sp. | diisopropyl phosphate + fluoride | - |
? | |
additional information | not: 4-nitrophenylphosphonate, 4-nitrophenylphosphinorylcholine | Alteromonas sp. | ? | - |
? | |
additional information | not: 4-nitrophenylphosphate | Alteromonas sp. | ? | - |
? | |
additional information | 4-nitrophenylacetate, bis(4-nitrophenyl)phosphate, tris(4-nitrophenyl)phosphate | Alteromonas sp. | ? | - |
? | |
sarin + H2O | at 20% the rate of diisopropyl fluorophosphate hydrolysis | Alteromonas sp. | ? | - |
? | |
soman + H2O | at 240% the rate of diisopropyl fluorophosphate hydrolysis | Alteromonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE, under reducing and nonreducing conditions | Alteromonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
3 mM diisopropyl fluorophosphate, pH 7.2, 1 mM MnCl2 | Alteromonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
583 | - |
diisopropyl fluorophosphate | pH 7.5, 22°C | Alteromonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
diisopropyl fluorophosphate | Alteromonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | 9 | about half-maximal activity at pH 7.6 and about 60% of maximal activity at pH 9 | Alteromonas sp. |