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Literature summary for 3.1.8.1 extracted from

  • Remy, B.; Plener, L.; Poirier, L.; Elias, M.; Daude, D.; Chabriere, E.
    Harnessing hyperthermostable lactonase from Sulfolobus solfataricus for biotechnological applications (2016), Sci. Rep., 6, 37780 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.8.1

Activating Compound

Activating Compound Comment Organism Structure
Methylcyclohexane about 250% activity at 100% (v/v) Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
W263I the mutant has increased lactonase and phosphotriesterase activities compared to the wild type Saccharolobus solfataricus

General Stability

General Stability Organism
with the liquid enzyme, the remaining activity decreases down to 40% at the highest dose of beta-radiation (100 kGy) Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
petroleum ether petroleum ether slightly reduces the activity down to 78% Saccharolobus solfataricus
Toluene toluene slightly reduces the activity down to 74% Saccharolobus solfataricus
xylene xylene decreases the enzyme activity by more than 75% Saccharolobus solfataricus

Organic Solvent Stability

Organic Solvent Comment Organism
additional information the enzyme activity is not affected by acetone, acetonitrile, butanone, butyl acetate, chloroform, dichloromethane, diethyl ether, ethanol, ethyl acetate, isopropanol, methanol, and methoxypropanol Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
 Sulfolobus solfataricus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethyl paraoxon + H2O
-
 Saccharolobus solfataricus ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 106°C is the melting temperature. The temperatures required to lose one half of enzymatic activity in 5 minutes are 92°C and 130°C in liquid and solid states, respectively. More than 30% activity remains after autoclaving the enzyme powder at 121°C for 15 min Saccharolobus solfataricus

General Information

General Information Comment Organism
metabolism the enzyme exhibits promiscuous phosphotriesterase activity for the degradation of organophosphorous chemicals including insecticides and chemical warfare agents Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2
-
 ethyl paraoxon at pH 8.0 and 23°C Saccharolobus solfataricus
1.4
-
 ethyl paraoxon with 50% (v/v) glycerol, at pH 8.0 and -18°C Saccharolobus solfataricus
25
-
 ethyl paraoxon at pH 8.0 and 70°C Saccharolobus solfataricus