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Literature summary for 3.1.8.1 extracted from
- High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and bioscavengers (2018), BMC Biotechnol., 18, 18 .
Application
| Application | Comment | Organism |
|---|---|---|
| environmental protection | thermostable phosphotriesterase-like lactonases (PLLs) are able to degrade organophosphates and can be potentially employed as bioremediation tools and bioscavengers | Sulfolobus acidocaldarius |
| environmental protection | thermostable phosphotriesterase-like lactonases (PLLs) are able to degrade organophosphates and can be potentially employed as bioremediation tools and bioscavengers. The enzyme is employable in cleaning organophosphates from different surfaces like glass, tissues, and fruits, also in presence of surfactants and even when dissolved in tap water | Saccharolobus solfataricus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of mutant enzyme SsoPox 3 M in Escherichia coli strain BL21(DE3), induction by galactose, method optimization | Saccharolobus solfataricus |
| recombinant expression of wild-type enzyme in Escherichia coli strain BL21(DE3), induction by galactose, method optimization | Sulfolobus acidocaldarius |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C258L/I261F/W263A | site-directed mutagenesis, mutant SsoPox 3 M | Saccharolobus solfataricus |
| additional information | optimization of enzyme production scale-up for establishment of an industrial purification process using SsoPox C258L/I261F/W263A mutant enzyme SsoPox 3 M, set up of high cell density fermentation strategies | Saccharolobus solfataricus |
| additional information | optimization of enzyme production scale-up for establishment of an industrial purification process using wild-type SacPox enzyme, set up of high cell density fermentation strategies. The enzyme is employable in cleaning organophosphates from different surfaces like glass, tissues, and fruits, also in presence of surfactants and even when dissolved in tap water | Sulfolobus acidocaldarius |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q97VT7 | ie. Saccharolobus solfataricus | - |
| Sulfolobus acidocaldarius | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) 7.63fold by heat treatment at 66-72°C, by response surface methodology, and ultrafiltration using an automatic tangential flow filtration system, followed by anion exchange chromatography. The ultrafiltration step is preferred compared to a gel filtration step | Sulfolobus acidocaldarius |
| recombinant enzyme mutant SsoPox 3 M from Escherichia coli strain BL21(DE3) 13.10fold by heat treatment at 66-72°C, by response surface methodology, and ultrafiltration using an automatic tangential flow filtration system, followed by anion exchange chromatography. The ultrafiltration step is preferred compared to a gel filtration step | Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.03 | - |
substrate paraoxon, wild-type enzyme, pH and temperature not specified in the publication | Sulfolobus acidocaldarius |
| 122.6 | - |
substrate paraoxon, enzyme mutant C258L/I261F/W263A, pH and temperature not specified in the publication | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diethyl-paraoxon + H2O | - |
Sulfolobus acidocaldarius | diethyl phosphate + 4-nitrophenol | - |
? |  |
| diethyl-parathion + H2O | - |
Sulfolobus acidocaldarius | diethyl thiophosphate + 4-nitrophenol | - |
? | |
| dimethyl-paraoxon + H2O | - |
Sulfolobus acidocaldarius | dimethyl phosphate + 4-nitrophenol | - |
? | |
| dimethyl-parathion + H2O | - |
Sulfolobus acidocaldarius | dimethyl thiophosphate + 4-nitrophenol | - |
? | |
| paraoxon + H2O | high activity with enzyme mutant C258L/I261F/W263A | Saccharolobus solfataricus | diethyl phosphate + 4-nitrophenol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phosphotriesterase-like lactonase | - |
Sulfolobus acidocaldarius |
| phosphotriesterase-like lactonase | - |
Saccharolobus solfataricus |
| PLL | - |
Sulfolobus acidocaldarius |
| PLL | - |
Saccharolobus solfataricus |
| SacPox | - |
Sulfolobus acidocaldarius |
| SsoPox | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 75 | - |
Sulfolobus acidocaldarius |
| 65 | 75 | enzyme mutant SsoPox 3 M | Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 100 | activity range | Sulfolobus acidocaldarius |
| 10 | 100 | activity range, enzyme mutant SsoPox 3 M | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Sulfolobus acidocaldarius |
| 8 | 8.5 | enzyme mutant SsoPox 3 M | Saccharolobus solfataricus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | activity range | Sulfolobus acidocaldarius |
| 5 | 9 | activity range, enzyme mutant SsoPox 3 M | Saccharolobus solfataricus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | thermostable phosphotriesterase-like lactonases (PLLs) are able to degrade organophosphates and act as bioscavengers | Sulfolobus acidocaldarius |
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