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Literature summary for 3.1.8.1 extracted from

  • Bigley, A.N.; Narindoshvili, T.; Xiang, D.F.; Raushel, F.M.
    Multiple reaction products from the hydrolysis of chiral and prochiral organophosphate substrates by the phosphotriesterase from Sphingobium sp. TCM1 (2018), Biochemistry, 57, 1842-1846 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.8.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 cyclohexyl methyl phenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
0.036
-
 methyl 4-nitrophenyl propan-2-yl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
0.053
-
 cyclohexyl methyl 4-nitrophenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
0.054
-
 cyclohexyl ethyl 4-nitrophenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
0.059
-
 cyclohexyl ethyl 4-nitrophenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
0.061
-
 methyl 4-nitrophenyl phenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
0.08
-
 methyl bis(4-nitrophenyl) phosphate pH 8.5, 30°C Sphingobium sp. TCM1
0.083
-
 diethyl 4-nitrophenyl phosphate pH 8.5, 30°C Sphingobium sp. TCM1
0.12
-
 4-acetylphenyl cyclohexyl methyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
0.25
-
 methyl 4-nitrophenyl phenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
0.31
-
 4-acetylphenyl cyclohexyl methyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
0.76
-
 dimethyl 4-nitrophenyl phosphate pH 8.5, 30°C Sphingobium sp. TCM1
1.1
-
 cyclohexyl methyl 4-nitrophenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
2.3
-
 methyl 4-nitrophenyl propan-2-yl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1

Metals/Ions

Metals/Ions Comment Organism Structure
additional information enzyme Sb-PTE uses a binuclear metal center Sphingobium sp. TCM1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sphingobium sp. TCM1 phosphotriesterase from Sphingobium sp. TCM1 (Sb-PTE) possesses a rather broad substrate profile and is able to hydrolyze insecticides, plasticizers, and flame retardants that are not typically substrates for other enzymes of this class ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingobium sp. TCM1 A0A077JBW9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-acetylphenyl cyclohexyl methyl (R)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl methyl (R)-phosphate + 4-acetylphenol
-
 ?
4-acetylphenyl cyclohexyl methyl (S)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl methyl (S)-phosphate + 4-acetylphenol
-
 ?
cyclohexyl ethyl 4-nitrophenyl (R)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl ethyl (R)-phosphate + 4-nitrophenol
-
 ?
cyclohexyl ethyl 4-nitrophenyl (S)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl ethyl (S)-phosphate + 4-nitrophenol
-
 ?
cyclohexyl methyl 4-nitrophenyl (R)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl methyl (R)-phosphate + 4-nitrophenol
-
 ?
cyclohexyl methyl 4-nitrophenyl (S)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl methyl (S)-phosphate + 4-nitrophenol
-
 ?
cyclohexyl methyl phenyl (R)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl methyl (R)-phosphate + phenol
-
 ?
cyclohexyl methyl phenyl (S)-phosphate + H2O
-
 Sphingobium sp. TCM1 cyclohexyl methyl (S)-phosphate + phenol
-
 ?
diethyl 4-nitrophenyl phosphate + H2O
-
 Sphingobium sp. TCM1 diethyl phosphate + 4-nitrophenol
-
 ?
dimethyl 4-nitrophenyl phosphate + H2O
-
 Sphingobium sp. TCM1 dimethyl phosphate + 4-nitrophenol
-
 ?
methyl 4-nitrophenyl phenyl (R)-phosphate + H2O
-
 Sphingobium sp. TCM1 methyl phenyl (R)-phosphate + 4-nitrophenol
-
 ?
methyl 4-nitrophenyl phenyl (S)-phosphate + H2O
-
 Sphingobium sp. TCM1 methyl phenyl (S)-phosphate + 4-nitrophenol
-
 ?
methyl 4-nitrophenyl propan-2-yl (R)-phosphate + H2O
-
 Sphingobium sp. TCM1 methyl propan-2-yl (R)-phosphate + 4-nitrophenol
-
 ?
methyl 4-nitrophenyl propan-2-yl (S)-phosphate + H2O
-
 Sphingobium sp. TCM1 methyl propan-2-yl (S)-phosphate + 4-nitrophenol
-
 ?
methyl bis(4-nitrophenyl) phosphate + H2O
-
 Sphingobium sp. TCM1 methyl phosphate + 2 4-nitrophenol
-
 ?
additional information phosphotriesterase from Sphingobium sp. TCM1 (Sb-PTE) possesses a rather broad substrate profile and is able to hydrolyze insecticides, plasticizers, and flame retardants that are not typically substrates for other enzymes of this class Sphingobium sp. TCM1 ?
-
 ?
additional information multiple reaction products from the hydrolysis of chiral and prochiral organophosphate substrates, substrate specificity and differential hydrolysis of the prochiral substituents, analysis of the enantioselectivity, overview Sphingobium sp. TCM1 ?
-
 ?

Synonyms

Synonyms Comment Organism
A7Q26_23485
-
 Sphingobium sp. TCM1
HAD
-
 Sphingobium sp. TCM1
haloalkylphosphorus hydrolase UniProt Sphingobium sp. TCM1
Sb-PTE
-
 Sphingobium sp. TCM1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Sphingobium sp. TCM1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.36
-
 4-acetylphenyl cyclohexyl methyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
0.38
-
 4-acetylphenyl cyclohexyl methyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
0.38
-
 cyclohexyl methyl phenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
0.6
-
 methyl 4-nitrophenyl phenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
1.11
-
 cyclohexyl methyl 4-nitrophenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
2.05
-
 methyl 4-nitrophenyl propan-2-yl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
2.5
-
 cyclohexyl ethyl 4-nitrophenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
2.8
-
 cyclohexyl ethyl 4-nitrophenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
5
-
 methyl 4-nitrophenyl propan-2-yl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
6
-
 cyclohexyl methyl 4-nitrophenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
6.3
-
 methyl 4-nitrophenyl phenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
6.5
-
 diethyl 4-nitrophenyl phosphate pH 8.5, 30°C Sphingobium sp. TCM1
9
-
 methyl bis(4-nitrophenyl) phosphate pH 8.5, 30°C Sphingobium sp. TCM1
9.1
-
 dimethyl 4-nitrophenyl phosphate pH 8.5, 30°C Sphingobium sp. TCM1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Sphingobium sp. TCM1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.16
-
 4-acetylphenyl cyclohexyl methyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
2.17
-
 methyl 4-nitrophenyl propan-2-yl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
2.4
-
 methyl 4-nitrophenyl phenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
3.17
-
 4-acetylphenyl cyclohexyl methyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
5.45
-
 cyclohexyl methyl 4-nitrophenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
11.97
-
 dimethyl 4-nitrophenyl phosphate pH 8.5, 30°C Sphingobium sp. TCM1
20.94
-
 cyclohexyl methyl 4-nitrophenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
42.37
-
 cyclohexyl ethyl 4-nitrophenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
51.85
-
 cyclohexyl ethyl 4-nitrophenyl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
56.94
-
 methyl 4-nitrophenyl propan-2-yl (S)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
63.33
-
 cyclohexyl methyl phenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
78.31
-
 diethyl 4-nitrophenyl phosphate pH 8.5, 30°C Sphingobium sp. TCM1
103.28
-
 methyl 4-nitrophenyl phenyl (R)-phosphate pH 8.5, 30°C Sphingobium sp. TCM1
112.5
-
 methyl bis(4-nitrophenyl) phosphate pH 8.5, 30°C Sphingobium sp. TCM1