Any feedback?
Literature summary for 3.1.8.1 extracted from
- Variants of phosphotriesterase for the enhanced detoxification of the chemical warfare agent VR (2015), Biochemistry, 54, 5502-5512 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Brevundimonas diminuta |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant enzyme mutants A80V/F132V/K185R/H254Q/H257Y/I274N/S308L and I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L, vapor diffusion method, mixing of 0.001 ml of 10 mg/ml protein in 50 mM HEPES, pH 8.5, and 1.0 mM CoCl2, with 0.001 ml of precipitant solution containing 100 mM sodium cacodylate, pH 5.5-7.0, 0.2 M magnesium acetate, and 15-30% PEG 8000, and equilbration against 0.5 ml of the precipitant solution, 7-21 days, 18°C, X-ray diffraction structure determination and analysis, molecular replacement using the coordinates of wild-type PTE, PDB ID 1DPM, as the search model | Brevundimonas diminuta |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A80V/F132V/K185R/H254Q/H257Y/I274N | site-directed mutagenesis | Brevundimonas diminuta |
| A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | site-directed mutagenesis, the mutant combines expression-enhancing mutations (A80V/K185R/I274N) with additional changes in the active site to achieve a high kcat/Km for the hydrolysis of the SP-enantiomer of ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX. Three-dimensional structure, overview | Brevundimonas diminuta |
| A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | site-directed mutagenesis | Brevundimonas diminuta |
| A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | site-directed mutagenesis | Brevundimonas diminuta |
| A80V/I106G/F132V/K185R/H254Q/H257Y/I274N | site-directed mutagenesis | Brevundimonas diminuta |
| A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | site-directed mutagenesis | Brevundimonas diminuta |
| F132V/H254Q/H257F | site-directed mutagenesis | Brevundimonas diminuta |
| F132V/H254Q/H257F/S308L | site-directed mutagenesis | Brevundimonas diminuta |
| H254Q/H257F | site-directed mutagenesis, the mutant exhibits a 100fold improvement for the hydrolysis of O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX, relative to the wild-type enzyme | Brevundimonas diminuta |
| I106C | site-directed mutagenesis | Brevundimonas diminuta |
| I106C/F132V/H254Q/H257F | site-directed mutagenesis | Brevundimonas diminuta |
| I106C/F132V/H254Q/H257F/S308L | site-directed mutagenesis, the mutant shows a similar catalytic efficiency for the hydrolysis of ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX, as mutant H254Q/H257F, but a 3fold improvement in kcat | Brevundimonas diminuta |
| I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | site-directed mutagenesis, the mutant has a kcat value for ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX, that is enhanced by 150fold relative to wild-type PTE. Three-dimensional structure, overview | Brevundimonas diminuta |
| I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F | site-directed mutagenesis | Brevundimonas diminuta |
| I106G | site-directed mutagenesis | Brevundimonas diminuta |
| I106G/F132V/H254Q/H257F/S308L | site-directed mutagenesis | Brevundimonas diminuta |
| I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | site-directed mutagenesis | Brevundimonas diminuta |
| L308S | site-directed mutagenesis | Brevundimonas diminuta |
| additional information | computational docking study of wild-type and mutant enzymes with the toxic substrates | Brevundimonas diminuta |
| Y309F | site-directed mutagenesis | Brevundimonas diminuta |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta |  |
| 0.0036 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 0.0053 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 0.0053 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 0.0054 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 0.0056 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 0.0067 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 0.0072 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 0.008 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 0.0092 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 0.0092 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F | Brevundimonas diminuta | |
| 0.011 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F | Brevundimonas diminuta | |
| 0.021 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 0.022 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 0.025 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 0.03 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 0.033 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 0.081 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 0.13 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 0.15 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 0.16 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 0.18 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 0.18 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 0.18 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 0.19 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 0.19 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 0.2 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 0.23 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 0.23 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 0.24 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 0.31 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 0.35 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 0.36 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 0.41 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 0.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 0.6 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 0.62 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 0.65 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 0.71 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 0.73 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 0.76 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 0.8 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 0.87 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 1 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F | Brevundimonas diminuta | |
| 1.2 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F | Brevundimonas diminuta | |
| 1.4 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 1.4 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 1.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 1.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 1.6 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 1.7 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 1.7 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 1.7 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 2 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 2.1 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 2.8 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 3.2 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 4 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 4.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates, required | Brevundimonas diminuta | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diethyl-paraoxon + H2O | Brevundimonas diminuta | - |
diethyl phosphate + 4-nitrophenol | - |
? | |
| O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate + H2O | Brevundimonas diminuta | i.e. VX | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevundimonas diminuta | P0A434 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes from cell-free enzyme extract by precipitation of nucleic acids by protamine sulfate, ammonium sulfate fractionation, and gel filtration, followed by anion exchange chromatography | Brevundimonas diminuta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-[2-(diethylamino)ethyl] O,O-diethyl phosphorothioate + H2O | - |
Brevundimonas diminuta | diethyl phosphate + 2-(diethylamino)ethane-1-thiol | - |
? | |
| (S)-[2-(diethylamino)ethyl] O,O-dimethyl phosphorothioate + H2O | - |
Brevundimonas diminuta | dimethyl phosphate + 2-(diethylamino)ethane-1-thiol | - |
? | |
| (S)-[2-(diethylamino)ethyl] O-(2-methylpropyl) methylphosphonothioate + H2O | - |
Brevundimonas diminuta | 2-methylpropyl methylphosphonate + 2-(diethylamino)ethane-1-thiol | - |
? | |
| (S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate + H2O | - |
Brevundimonas diminuta | diethyl phosphate + 2-[di(propan-2-yl)amino]ethane-1-thiol | - |
? | |
| (S)-[2-[di(propan-2-yl)amino]ethyl] O,O-dimethyl phosphorothioate + H2O | - |
Brevundimonas diminuta | dimethyl phosphate + 2-[di(propan-2-yl)amino]ethane-1-thiol | - |
? | |
| (S)-[2-[di(propan-2-yl)amino]ethyl] O-ethyl methylphosphonothioate + H2O | - |
Brevundimonas diminuta | ethyl methylphosphonate + [di(propan-2-yl)amino]ethane-1-thiol | - |
? | |
| 4-acetylphenyl (R)-2-methylpropyl methylphosphonate + H2O | - |
Brevundimonas diminuta | (R)-2-methylpropyl methylphosphonate + 4-acetylphenol | - |
? | |
| 4-acetylphenyl (S)-2-methylpropyl methylphosphonate + H2O | - |
Brevundimonas diminuta | (S)-2-methylpropyl methylphosphonate + 4-acetylphenol | - |
? | |
| diethyl-paraoxon + H2O | - |
Brevundimonas diminuta | diethyl phosphate + 4-nitrophenol | - |
? | |
| additional information | wild-type PTE exhibits little stereoselectivity against the relatively small phosphonate center of O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX, but the elevated pKa of the thiol-leaving group provides a significant challenge for enzyme-catalyzed hydrolysis. Wild-type PTE has enzymatic activity for the hydrolysis of racemic VR similar to VX, but the larger isobutyl group attached to the phosphorus center results in a 25fold preference for the RP-enantiomer. Computational docking study of wild-type and mutant enzymes with the substrates | Brevundimonas diminuta | ? | - |
? | |
| N,N-diethyl-2-(methyl-(2-methylpropoxy)phosphoryl)sulfanylethanamine + H2O | i.e. VR | Brevundimonas diminuta | ? | - |
? | |
| O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate + H2O | i.e. VX | Brevundimonas diminuta | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phosphotriesterase | - |
Brevundimonas diminuta |
| PTE | - |
Brevundimonas diminuta |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Brevundimonas diminuta |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 3 | 6 | (S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F | Brevundimonas diminuta | |
| 3 | 6 | 4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 5.1 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 6.1 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 8.1 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 8.6 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 8.7 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 13 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 14 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 14 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 15 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 16 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 17 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 19 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F | Brevundimonas diminuta | |
| 21 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 22 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 23 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 25 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 29 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 31 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 33 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 33 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 35 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 38 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 40 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 41 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 45 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 46 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 48 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 50 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 51 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 51 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 53 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 55 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 56 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 57 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 58 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 58 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 66 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 72 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 84 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 85 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 100 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 101 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 103 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 108 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F | Brevundimonas diminuta | |
| 116 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 122 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 131 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F | Brevundimonas diminuta | |
| 159 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 160 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 166 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 174 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 446 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 456 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 545 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 2230 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | assay at | Brevundimonas diminuta |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.264 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 4.0625 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 4.357 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 5.375 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 5.55 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 9.3 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 11.071 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 14.571 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 17.1 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 19 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F | Brevundimonas diminuta | |
| 21.053 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 21.4 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 21.539 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 25 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 28.75 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 30 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F | Brevundimonas diminuta | |
| 30.137 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 32.7 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 34 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 34.375 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 38.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 42.6 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 49.4 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
| 61 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 81.7 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 85 | - |
(S)-[2-[di(propan-2-yl)amino]ethyl] O,O-diethyl phosphorothioate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 94.1 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 110.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 112 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 124.3 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 143.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 158.3 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 162.9 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 166.67 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 180 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 255.6 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 290 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 294.4 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 313 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 333.3 | - |
4-acetylphenyl (R)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 535.5 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 883.3 | - |
4-acetylphenyl (S)-2-methylpropyl methylphosphonate | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 1920 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 4925 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F | Brevundimonas diminuta | |
| 6304 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 6786 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 7736 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant H254Q/H257F | Brevundimonas diminuta | |
| 8824 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 9722 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N | Brevundimonas diminuta | |
| 9818 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F | Brevundimonas diminuta | |
| 11806 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 12222 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 14239 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106C/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L/Y309F | Brevundimonas diminuta | |
| 14500 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 16515 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257Y/A270V/L272M/I274N/S308L | Brevundimonas diminuta | |
| 19434 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106C/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 20727 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant I106G/F132V/H254Q/H257F/S308L | Brevundimonas diminuta | |
| 21239 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant mutant A80V/I106G/F132V/K185R/H254Q/H257Y/I274N/S308L | Brevundimonas diminuta | |
| 27531 | - |
diethyl-paraoxon | pH 9.0, 30°C, recombinant wild-type enzyme | Brevundimonas diminuta | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
