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Literature summary for 3.1.8.1 extracted from
- Extensive hydrolysis of phosphonates as unexpected behaviour of the known His6-organophosphorus hydrolase (2016), Appl. Microbiol. Biotechnol., 100, 5829-5838 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PON1, recombinant expression of His6-tagged enzyme in Escherichia coli strain SG13009[pREP4] | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | nanocapsulation of enzyme into enzyme-polyelectrolyte complexes leads to its expected stabilisation and significant improvement of Km and Ksi with methylphosphonic acid | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| nanocapsulation of enzyme into enzyme-polyelectrolyte complexes leads to its expected stabilisation and significant improvement of Km and Ksi with methylphosphonic acid | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methylphosphonic acid | possibly inhibits the enzyme, substrate inhibition | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.6 | - |
O,O'-(diisobutyl)methylphosphonate | pH 10.5, 25°C, recombinant His6-tagged enzyme | Homo sapiens | |
| 1400 | - |
O-(isobutyl)methylphosphonate | pH 10.5, 25°C, recombinant His6-tagged enzyme | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates, required | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diethyl-paraoxon + H2O | Homo sapiens | - |
diethyl phosphate + 4-nitrophenol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P27169 | - |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| an aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol | hypothetic mechanism of C-P bond cleavage in methylphosphonic acid by recombinant His6-tagged OPH, overview | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| serum | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diethyl-paraoxon + H2O | - |
Homo sapiens | diethyl phosphate + 4-nitrophenol | - |
? | |
| methylphosphonic acid + H2O | i.e. MPA, according to the C-P lyase pathway, methylphosphonic acid decomposition by the enzyme is expected to yield methane as a product. But no methane is detected during the reaction process. Methanol cannot be detected either during the MPA decomposition reaction, as well as formaldehyde, but formic acid is identified in the reaction mixture | Homo sapiens | ? | - |
? | |
| additional information | the recombinant His6-tagged organophosphorus hydrolase (His6-OPH) shows catalytic activity in hydrolytic reactions with methylphosphonic acid (MPA) monoesters and diesters being decomposition products of R-VX nerve agent derivative (O-ethyl-S-(2-diisopropylaminoethyl)methylphosphonothiolate, i.e. VX), analysis of the mechanism of C-P bond cleavage in methylphosphonic acid by His6-OPH, overview. The recombinant enzyme His6-OPH is capable of degrading the key organophosphorus components of reaction masses (RMs) that are produced by chemical detoxification of R-VX, the RMs are multisubstrate mixtures for this enzyme, R-VX decomposition scheme. GC-MS analysis of phosphonates and methane | Homo sapiens | ? | - |
? | |
| O,O'-(diisobutyl)methylphosphonate + H2O | - |
Homo sapiens | ? | - |
? | |
| O-(isobutyl)methylphosphonate + H2O | very low activity | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| OPH | - |
Homo sapiens |
| organophosphorus hydrolase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 46 | 48 | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
the maximum rate of methylphosphonic acid hydrolysis is achieved at pH 10.5. This corresponds to the pH optimum of the enzyme activity in the reaction of paraoxon hydrolysis and contributes to the formation of activated C-P bond, which is required for methylphosphonic acid hydrolysis | Homo sapiens |
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