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Literature summary for 3.1.8.1 extracted from
- Cloning of an organophosphorus hydrolase (opdD) gene of Lactobacillus sakei WCP904 isolated from chlorpyrifos-impregnated kimchi and hydrolysis activities of its gene product for organophosphorus pesticides (2018), Appl. Biol. Chem., 61, 643-651 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene opdD, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain BL21(DE3) | Latilactobacillus sakei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cadusafos + H2O | Latilactobacillus sakei | - |
O,O-diethylphosphorodithioate + phenol | - |
? |  |
| cadusafos + H2O | Latilactobacillus sakei WCP904 | - |
O,O-diethylphosphorodithioate + phenol | - |
? | |
| chlorpyrifos + H2O | Latilactobacillus sakei | - |
O,O-diethylphosphorothioate + 3,5,6-trichloropyridin-2-ol | - |
? | |
| chlorpyrifos + H2O | Latilactobacillus sakei WCP904 | - |
O,O-diethylphosphorothioate + 3,5,6-trichloropyridin-2-ol | - |
? | |
| coumaphos + H2O | Latilactobacillus sakei | - |
O,O-diethylphosphorothioate + 3-chloro-4-methylumbelliferone | - |
? | |
| coumaphos + H2O | Latilactobacillus sakei WCP904 | - |
O,O-diethylphosphorothioate + 3-chloro-4-methylumbelliferone | - |
? | |
| diazinon + H2O | Latilactobacillus sakei | - |
O,O-diethylphosphorothioate + ? | - |
? | |
| diazinon + H2O | Latilactobacillus sakei WCP904 | - |
O,O-diethylphosphorothioate + ? | - |
? | |
| diethyl-parathion + H2O | Latilactobacillus sakei | - |
diethyl thiophosphate + 4-nitrophenol | - |
? | |
| dimethyl-parathion + H2O | Latilactobacillus sakei | - |
dimethyl thiophosphate + 4-nitrophenol | - |
? | |
| dyfonate + H2O | Latilactobacillus sakei | - |
O,O-diethylphosphorothioate + 2-isopropyl-6-methylpyrimidin-4-ol | - |
? | |
| ethoprophos + H2O | Latilactobacillus sakei | - |
? | - |
? | |
| fenamiphos + H2O | Latilactobacillus sakei | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Latilactobacillus sakei | - |
isolated from kimchi fermentation, yeolmu-mulkimchi contaminated with chlorpyrifos | - |
| Latilactobacillus sakei WCP904 | - |
isolated from kimchi fermentation, yeolmu-mulkimchi contaminated with chlorpyrifos | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) | Latilactobacillus sakei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity in wild-type strain WCP904 and of purified recombinant enzyme with different organophosphorus pesticides, overview | Latilactobacillus sakei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butyrate + H2O | - |
Latilactobacillus sakei | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Latilactobacillus sakei WCP904 | 4-nitrophenol + butyrate | - |
? | |
| cadusafos + H2O | - |
Latilactobacillus sakei | O,O-diethylphosphorodithioate + phenol | - |
? | |
| cadusafos + H2O | - |
Latilactobacillus sakei WCP904 | O,O-diethylphosphorodithioate + phenol | - |
? | |
| chlorpyrifos + H2O | - |
Latilactobacillus sakei | O,O-diethylphosphorothioate + 3,5,6-trichloropyridin-2-ol | - |
? | |
| chlorpyrifos + H2O | - |
Latilactobacillus sakei WCP904 | O,O-diethylphosphorothioate + 3,5,6-trichloropyridin-2-ol | - |
? | |
| coumaphos + H2O | - |
Latilactobacillus sakei | O,O-diethylphosphorothioate + 3-chloro-4-methylumbelliferone | - |
? | |
| coumaphos + H2O | - |
Latilactobacillus sakei WCP904 | O,O-diethylphosphorothioate + 3-chloro-4-methylumbelliferone | - |
? | |
| diazinon + H2O | - |
Latilactobacillus sakei | O,O-diethylphosphorothioate + ? | - |
? | |
| diazinon + H2O | - |
Latilactobacillus sakei WCP904 | O,O-diethylphosphorothioate + ? | - |
? | |
| diethyl-parathion + H2O | - |
Latilactobacillus sakei | diethyl thiophosphate + 4-nitrophenol | - |
? | |
| dimethyl-parathion + H2O | - |
Latilactobacillus sakei | dimethyl thiophosphate + 4-nitrophenol | - |
? | |
| dyfonate + H2O | - |
Latilactobacillus sakei | O,O-diethylphosphorothioate + 2-isopropyl-6-methylpyrimidin-4-ol | - |
? | |
| ethoprophos + H2O | - |
Latilactobacillus sakei | ? | - |
? | |
| fenamiphos + H2O | - |
Latilactobacillus sakei | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31000, recombinant enzyme, SDS-PAGE | Latilactobacillus sakei |
| More | the deduced amino acid sequences of OpdD shows a potential serine hydrolase motif such as G-H-S116-Q-G | Latilactobacillus sakei |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| OpdD | - |
Latilactobacillus sakei |
| organophosphorus hydrolase | - |
Latilactobacillus sakei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
recombinant enzyme, with substrate 4-nitrophenyl butyrate | Latilactobacillus sakei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
recombinant enzyme, with substrate 4-nitrophenyl butyrate | Latilactobacillus sakei |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the deduced amino acid sequences of OpdD show a potential serine hydrolase motif such as G-H-S116-Q-G | Latilactobacillus sakei |
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