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Literature summary for 3.1.8.1 extracted from

  • Jeong, Y.S.; Choi, J.M.; Kyeong, H.H.; Choi, J.Y.; Kim, E.J.; Kim, H.S.
    Rational design of organophosphorus hydrolase with high catalytic efficiency for detoxifying a V-type nerve agent (2014), Biochem. Biophys. Res. Commun., 449, 263-267.
    View publication on PubMed
Search for more literature for 3.1.8.1

Protein Variants

Protein Variants Comment Organism
A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S mutant constructed fo expression of soluble and active enzyme Flavobacterium sp.
L271/Y309A mutant based on expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, 2fold increase in reactivity with substrate demeton-S-methyl Flavobacterium sp.
L271A mutant based on expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, similar soluble expression Flavobacterium sp.
L271A/Y309A mutant based on expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, exhibits a 150fold higher catalytic efficiency for V-type nerve agents such as S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate than the wild-type Flavobacterium sp.
T172A/L271A mutant based on expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, decrease in activity Flavobacterium sp.
T172A/L271A/Y309A mutant based on expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, decrease in activity Flavobacterium sp.
T172A/Y309A mutant based on expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, 2fold increase in reactivity with substrate demeton-S-methyl Flavobacterium sp.
Y309A mutant based on expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, increase in soluble expression Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, pH 8.5, temperature not specified in the publication Flavobacterium sp.
5.87
-
 S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate mutant L271A/Y309A, pH 8.5, temperature not specified in the publication Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
demeton-S-methyl + H2O
-
 Flavobacterium sp. 2-ethylsulfanyl-ethanethiol + dimethyl phosphate
-
 ?
S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate i.e. nerve agent VX Flavobacterium sp. O-ethyl hydrogen methylphosphonothioate + 2-[di(propan-2-yl)amino]ethanol
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.007
-
 S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, pH 8.5, temperature not specified in the publication Flavobacterium sp.
0.15
-
 S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate mutant L271A/Y309A, pH 8.5, temperature not specified in the publication Flavobacterium sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00486
-
 S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate expression mutant A80V/I106V/F132D/K185R/D208G/H257W/I274N/S308L/R319S, pH 8.5, temperature not specified in the publication Flavobacterium sp.
0.0249
-
 S-(2-[di(propan-2-yl)amino]ethyl)O-ethyl methylphosphonothioate mutant L271A/Y309A, pH 8.5, temperature not specified in the publication Flavobacterium sp.