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Literature summary for 3.1.8.1 extracted from

  • Porzio, E.; Di, Gennaro, S.; Palma, A.; Manco, G.
    Mn2+ modulates the kinetic properties of an archaeal member of the PLL family (2013), Chem. Biol. Interact., 203, 251-256.
    View publication on PubMed
Search for more literature for 3.1.8.1

Cloned(Commentary)

Cloned (Comment) Organism
SacPox is expressed in the presence of 0.2 mM of each of the following salts: CdCl2, CoCl2, MnCl2, ZnCl2, NiSO4. The gene sacpox is cloned in the expression vector pT7-7, and overexpressed in Escherichia coli BL21(DE3) cells. The highest activity is obtained for the crude extract of a Mn2+-enriched culture Sulfolobus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.138
-
 methyl paraoxon pH 8.5, 70°C, Mn2+-containing enzyme Sulfolobus acidocaldarius
0.32
-
 paraoxon pH 8.5, 70°C, Mn2+-containing enzyme Sulfolobus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ the Mn2+-containing enzyme is about 30-fold more efficient with paraoxon as substrate and more stable than the Cd2+ counterpart, even though the Mn2+ affinity for the binuclear metal centre is apparently lower Sulfolobus acidocaldarius
Mn2+ the Mn2+-containing enzyme is about 30-fold more efficient with paraoxon as substrate and more stable than the Cd2+ counterpart, even though the Mn2+ affinity for the binuclear metal centre is apparently lower Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfolobus acidocaldarius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.3
-
 substrate: paraoxon, pH 8.5, 70°C, Mn2+-containing enzyme Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl paraoxon + H2O O,O-dimethyl O-4-nitrophenyl phosphate Sulfolobus acidocaldarius 4-nitrophenol + dimethyl phosphate
-
 ?
paraoxon + H2O i.e. diethyl-4-nitrophenyl-phosphate Sulfolobus acidocaldarius 4-nitrophenol + diethyl phosphate
-
 ?

Synonyms

Synonyms Comment Organism
SacPox
-
 Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 Mn2+-containing enzyme Sulfolobus acidocaldarius

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 90 50°C: about 50% of maximal activity, 90°C: about 80% of maximal activity, Mn2+-containing enzyme Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 t1/2: 3.5 h, Mn2+-containing enzyme Sulfolobus acidocaldarius
80
-
 t1/2: 3 h, Mn2+-containing enzyme Sulfolobus acidocaldarius
90
-
 t1/2: 2 h, Mn2+-containing enzyme Sulfolobus acidocaldarius

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.52
-
 paraoxon pH 8.5, 70°C, Mn2+-containing enzyme Sulfolobus acidocaldarius
14.05
-
 methyl paraoxon pH 8.5, 70°C, Mn2+-containing enzyme Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 Mn2+-containing enzyme Sulfolobus acidocaldarius
9
-
 Cd2+-containing enzyme Sulfolobus acidocaldarius

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 pH 7.0: about 55% of maximal activity, pH 9.5: about 85% of maximal activity, Cd2+-containing enzyme Sulfolobus acidocaldarius
7.5 9 pH 7.5: about 65% of maximal activity, pH 9.0: about 75% of maximal activity, Mn2+-containing enzyme Sulfolobus acidocaldarius

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
26.6
-
 paraoxon pH 8.5, 70°C, Mn2+-containing enzyme Sulfolobus acidocaldarius
102
-
 methyl paraoxon pH 8.5, 70°C, Mn2+-containing enzyme Sulfolobus acidocaldarius