Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SDS | 0.1% (w/v), 25°C, wild type enzyme, 11-fold increase of specific activity, activation is stable up to 40 h. The activating effect is reduced at 70°C | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
W263F | kcat/Km for paraoxon is 6.5fold higher than wild-type value | Saccharolobus solfataricus |
W263Q | kcat/Km for paraoxon is 3.5fold lower than wild-type value | Saccharolobus solfataricus |
Y97W | kcat/Km for paraoxon is 2.4fold higher than wild-type value | Saccharolobus solfataricus |
Y97W/I261F | kcat/Km for paraoxon is 1.4fold higher than wild-type value | Saccharolobus solfataricus |
Y97W/I98F/I261F | kcat/Km for paraoxon is 1.2fold higher than wild-type value | Saccharolobus solfataricus |
Y97W/W263F | kcat/Km for paraoxon is 1.6fold higher than wild-type value | Saccharolobus solfataricus |
Y97W/W263Q | kcat/Km for paraoxon is 1.7fold lower than wild-type value | Saccharolobus solfataricus |
General Stability | Organism |
---|---|
stable for up to 40 h in 0.2% SDS at 25°C | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.064 | - |
paraoxon | pH 8.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.075 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme Y97W | Saccharolobus solfataricus | |
0.153 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme W263F | Saccharolobus solfataricus | |
0.272 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme Y97W/W263F | Saccharolobus solfataricus | |
0.41 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme Y97W/I261F | Saccharolobus solfataricus | |
1.2 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme Y97W/I98F/I261F | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97VT7 | - |
- |
Saccharolobus solfataricus P2 | Q97VT7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-cyclosarin + H2O | wild type enzyme and mutant enzyme W263F hydrolyze the (+)-enantiomer approximately 3 and 4.5 times faster than the (-)-enantiomer | Saccharolobus solfataricus | methyl-phosphonic acid monofluoride + cyclohexanol | - |
? | |
(+)-cyclosarin + H2O | wild type enzyme and mutant enzyme W263F hydrolyze the (+)-enantiomer approximately 3 and 4.5 times faster than the (-)-enantiomer | Saccharolobus solfataricus P2 | methyl-phosphonic acid monofluoride + cyclohexanol | - |
? | |
paraoxon + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + diethyl phosphate | - |
? | |
paraoxon + H2O | - |
Saccharolobus solfataricus P2 | 4-nitrophenol + diethyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
phosphotriesterase-like lactonase | - |
Saccharolobus solfataricus |
SSO2522 | locus name | Saccharolobus solfataricus |
SsoPox | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
assay at | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
paraoxon | pH 8.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
0.68 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme Y97W | Saccharolobus solfataricus | |
1.63 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme Y97W/W263F | Saccharolobus solfataricus | |
2.2 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme Y97W/I261F | Saccharolobus solfataricus | |
3.7 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme Y97W/I98F/I261F | Saccharolobus solfataricus | |
3.76 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme W263F | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.07 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme W263Q | Saccharolobus solfataricus | |
2.2 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme Y97W/W263Q | Saccharolobus solfataricus | |
2.45 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme W263F | Saccharolobus solfataricus | |
3.05 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme Y97W/I98F/I261F | Saccharolobus solfataricus | |
3.75 | - |
paraoxon | pH 8.0, 70°C, wild-type enzyme | Saccharolobus solfataricus | |
5.4 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme Y97W/I261F | Saccharolobus solfataricus | |
6.02 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme Y97W/W263F | Saccharolobus solfataricus | |
9.01 | - |
paraoxon | pH 8.0, 70°C, mutant enzyme Y97W | Saccharolobus solfataricus |