Cloned (Comment) | Organism |
---|---|
expressed in Trichoplusia ni larvae | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
diethyl paraoxon | recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
0.772 | - |
diethyl paraoxon | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
0.889 | - |
diethyl paraoxon | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Homo sapiens | |
Ca2+ | required | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Cibacron Blue 3GA-agarose column chromatography, DEAE-Sepharose column chromatography, gel filtration, and Sepharose CL-6B column chromatography | Homo sapiens |
Cibacron Blue 3GA-agarose column chromatography, DEAE-Sepharose column chromatography, gel filtration, and Sepharose CL-6B column chromatography | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
serum | - |
Homo sapiens | - |
serum | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diethyl paraoxon + H2O | - |
Homo sapiens | ? | - |
? | |
diethyl paraoxon + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
diisopropyl fluorophosphate + H2O | high concentration of purified human serum paraoxonase 1 (about 0.075 mg) is required to hydrolyze 0.002 mM diisopropyl fluorophosphates | Homo sapiens | ? | - |
? | |
diisopropyl fluorophosphate + H2O | high concentration of purified rabbit serum paraoxonase 1 (about 0.025 mg) is required to hydrolyze 0.002 mM diisopropyl fluorophosphates | Oryctolagus cuniculus | ? | - |
? | |
dimethyl paraoxon + H2O | - |
Homo sapiens | ? | - |
? | |
dimethyl paraoxon + H2O | - |
Oryctolagus cuniculus | ? | - |
? | |
additional information | recombinant human paraoxoanase 1 cannot hydrolyze dimethyl paraoxon even at higher concentrations used | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000-45000, rabbit serum purified paraoxonase 1 shows three bands in about 40000-45000 Da molecular weight regions, SDS-PAGE | Oryctolagus cuniculus |
? | x * 42000-45000, purified recombinant human paraoxoanase 1 shows three bands at about 42000-45000 Da range, SDS-PAGE | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.3 | - |
diethyl paraoxon | recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
5.7 | - |
diethyl paraoxon | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
16.2 | - |
diethyl paraoxon | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Oryctolagus cuniculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
diethyl paraoxon | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Oryctolagus cuniculus | |
6.5 | - |
diethyl paraoxon | recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens | |
7.3 | - |
diethyl paraoxon | in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication | Homo sapiens |