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Literature summary for 3.1.8.1 extracted from

  • Valiyaveettil, M.; Alamneh, Y.; Biggemann, L.; Soojhawon, I.; Farag, H.A.; Agrawal, P.; Doctor, B.P.; Nambiar, M.P.
    In vitro efficacy of paraoxonase 1 from multiple sources against various organophosphates (2011), Toxicol. In Vitro, 25, 905-913.
    View publication on PubMed
Search for more literature for 3.1.8.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Trichoplusia ni larvae Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
 diethyl paraoxon recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
0.772
-
 diethyl paraoxon in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
0.889
-
 diethyl paraoxon in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Homo sapiens
Ca2+ required Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Cibacron Blue 3GA-agarose column chromatography, DEAE-Sepharose column chromatography, gel filtration, and Sepharose CL-6B column chromatography Homo sapiens
Cibacron Blue 3GA-agarose column chromatography, DEAE-Sepharose column chromatography, gel filtration, and Sepharose CL-6B column chromatography Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
 Homo sapiens
-
serum
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diethyl paraoxon + H2O
-
 Homo sapiens ?
-
 ?
diethyl paraoxon + H2O
-
 Oryctolagus cuniculus ?
-
 ?
diisopropyl fluorophosphate + H2O high concentration of purified human serum paraoxonase 1 (about 0.075 mg) is required to hydrolyze 0.002 mM diisopropyl fluorophosphates Homo sapiens ?
-
 ?
diisopropyl fluorophosphate + H2O high concentration of purified rabbit serum paraoxonase 1 (about 0.025 mg) is required to hydrolyze 0.002 mM diisopropyl fluorophosphates Oryctolagus cuniculus ?
-
 ?
dimethyl paraoxon + H2O
-
 Homo sapiens ?
-
 ?
dimethyl paraoxon + H2O
-
 Oryctolagus cuniculus ?
-
 ?
additional information recombinant human paraoxoanase 1 cannot hydrolyze dimethyl paraoxon even at higher concentrations used Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000-45000, rabbit serum purified paraoxonase 1 shows three bands in about 40000-45000 Da molecular weight regions, SDS-PAGE Oryctolagus cuniculus
? x * 42000-45000, purified recombinant human paraoxoanase 1 shows three bands at about 42000-45000 Da range, SDS-PAGE Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3
-
 diethyl paraoxon recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
5.7
-
 diethyl paraoxon in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
16.2
-
 diethyl paraoxon in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Oryctolagus cuniculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.8
-
 diethyl paraoxon in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Oryctolagus cuniculus
6.5
-
 diethyl paraoxon recombinant enzyme, in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens
7.3
-
 diethyl paraoxon in 20 mM Tris-HCl buffer, pH 7.4, 1 mM CaCl2, temperature not specified in the publication Homo sapiens