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Literature summary for 3.1.8.1 extracted from

  • Chungjatupornchai, W.; Fa-Aroonsawat, S.
    Biodegradation of organophosphate pesticide using recombinant Cyanobacteria with surface- and intracellular-expressed organophosphorus hydrolase (2008), J. Microbiol. Biotechnol., 18, 946-951.
    View publication on PubMed
Search for more literature for 3.1.8.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Synechococcus PCC7942 strain R2-SPc Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.345
-
 paraoxon surface-displayed recombinant enzyme from fresh cells, in 50 mM CHES/CoCl2 buffer, pH 9.0, with 0.05 mM CoCl2, at 37°C Flavobacterium sp.
0.347
-
 paraoxon surface-displayed recombinant enzyme from freeze-thawed cells, in 50 mM CHES/CoCl2 buffer, pH 9.0, with 0.05 mM CoCl2, at 37°C Flavobacterium sp.
1.047
-
 paraoxon intracellular-expressed recombinant enzyme from fresh cells, in 50 mM CHES/CoCl2 buffer, pH 9.0, with 0.05 mM CoCl2, at 37°C Flavobacterium sp.
1.051
-
 paraoxon intracellular expressed recombinant enzyme from freeze-thawed cells, in 50 mM CHES/CoCl2 buffer, pH 9.0, with 0.05 mM CoCl2, at 37°C Flavobacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the enzyme is displayed on cell surface Flavobacterium sp. 16020
-

Organism

Organism UniProt Comment Textmining
Flavobacterium sp. P0A433 strain ATCC 27551
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
paraoxon + H2O
-
 Flavobacterium sp. diethylphosphate + 4-nitrophenol
-
 ?

Synonyms

Synonyms Comment Organism
OPH
-
 Flavobacterium sp.
organophosphorus hydrolase
-
 Flavobacterium sp.
parathion hydrolase
-
 Flavobacterium sp.