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Literature summary for 3.1.8.1 extracted from

  • Kanamori-Kataoka, M.; Seto, Y.
    Paraoxonase activity against nerve gases measured by capillary electrophoresis and characterization of human serum paraoxonase (PON1) polymorphism in the coding region (Q192R) (2009), Anal. Biochem., 385, 94-100.
    View publication on PubMed
Search for more literature for 3.1.8.1

Protein Variants

Protein Variants Comment Organism
Q192R the mutant shows lower Vmax and kcat/Km values for soman and sarin than the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.074
-
 sarin mutant enzyme Q192R, in 20 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens
0.2
-
 O-ethyl S-2-diisopropylaminoethyl methylphosphonothiolate mutant enzyme Q192R, in 20 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens
0.2
-
 O-ethyl S-2-diisopropylaminoethyl methylphosphonothiolate wild type enzyme, in 20 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens
0.21
-
 sarin wild type enzyme, in 20 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens
0.27
-
 soman wild type enzyme, in 20 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens
0.44
-
 soman mutant enzyme Q192R, in 20 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens
0.94
-
 paraoxon wild type enzyme, in 50 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens
1.2
-
 paraoxon mutant enzyme Q192R, in 50 mM Tris-HCl with 1 mM CaCl2 (pH 7.4), at 25°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P27169
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
blue agarose affinity chromatography and HiTrap DEAE column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0012
-
 wild type enzyme from crude extract, using paraoxon as substrate Homo sapiens
0.0013
-
 mutant enzyme Q192R from crude extract, using paraoxon as substrate Homo sapiens
0.94
-
 mutant enzyme Q192R after 1018fold purification, using paraoxon as substrate Homo sapiens
1.21
-
 wild type enzyme after 1018fold purification, using paraoxon as substrate Homo sapiens

Storage Stability

Storage Stability Organism
4°C, purified PON1 in DEAE buffer, at least two weeks, remains stable Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chlorpyrifos + H2O
-
 Homo sapiens ?
-
 ?
chlortion + H2O chlorthion is O,O-dimethyl O-(3-chloro-4-nitrophenyl) thionophosphate Homo sapiens ?
-
 ?
O-ethyl O-4-nitrophenyl phenylphosphonothioate + H2O
-
 Homo sapiens ?
-
 ?
O-ethyl S-2-diisopropylaminoethyl methylphosphonothiolate + H2O
-
 Homo sapiens ?
-
 ?
paraoxon + H2O
-
 Homo sapiens diethylphosphate + 4-nitrophenol
-
 ?
sarin + H2O PON1 hydrolyzes sarin more effectively than paraoxon, sarin is o-isopropyl methylphosphonofluoridate Homo sapiens methyl-phosphonic acid monofluoride + isopropyl alcohol
-
 ?
soman + H2O PON1 hydrolyzes soman more effectively than paraoxon, soman is o-pinacolyl methylphosphonofluoridate Homo sapiens methylphosphonofluoride acid + 3,3-dimethylbutan-2-ol
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
paraoxonase
-
 Homo sapiens
PON1 the calcium-dependent glycoprotein is associated with high-density lipoprotein Homo sapiens