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Literature summary for 3.1.8.1 extracted from

  • Chu, X.Y.; Wu, N.F.; Deng, M.J.; Tian, J.; Yao, B.; Fan, Y.L.
    Expression of organophosphorus hydrolase OPHC2 in Pichia pastoris: purification and characterization (2006), Protein Expr. Purif., 49, 9-14.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
ORF ophc2, subcloning in Escherichia coli strain JM109, functional high level expression of OPHC2 in Pichia pastoris strain GS115, secretion to the culture supernatant Pseudomonas oleovorans

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 5% inhibition at 1 mM Pseudomonas oleovorans
Mn2+ 18% inhibition at 1 mM Pseudomonas oleovorans
Pb2+ 20% inhibition at 1 mM Pseudomonas oleovorans
Zn2+ 11% inhibition at 1 mM Pseudomonas oleovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates by 14.2% at 1 mM Pseudomonas oleovorans
additional information no effect by Ca2+, Cr3+, Mg2+, Li+, and EDTA at 1 mM Pseudomonas oleovorans
Ni2+ activates by 23.5% at 1 mM Pseudomonas oleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
x * 36000, recombinant OPHC2, SDS-PAGE Pseudomonas oleovorans

Organism

Organism UniProt Comment Textmining
Pseudomonas oleovorans Q5W503 ORF ophc2
-
Pseudomonas oleovorans C2-1 Q5W503 ORF ophc2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant OPHC2 from Pichia pastoris strain GS115 culture supernatant by gel filtration and ultrafiltration Pseudomonas oleovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl parathion + H2O
-
Pseudomonas oleovorans dimethyl thiophosphate + 4-nitrophenol
-
?
methyl parathion + H2O
-
Pseudomonas oleovorans C2-1 dimethyl thiophosphate + 4-nitrophenol
-
?

Subunits

Subunits Comment Organism
? x * 36000, recombinant OPHC2, SDS-PAGE Pseudomonas oleovorans

Synonyms

Synonyms Comment Organism
OPHC2
-
Pseudomonas oleovorans
organophosphorus hydrolase
-
Pseudomonas oleovorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
recombinant enzyme Pseudomonas oleovorans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80
-
Pseudomonas oleovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
30 min, purified recombinant enzyme, over 60% remaining activity Pseudomonas oleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
recombinant enzyme Pseudomonas oleovorans

pH Range

pH Minimum pH Maximum Comment Organism
5 10 50% of maximal activity at pH 5.0 and below Pseudomonas oleovorans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
30 min, purified enzyme, loss of 50% activity at pH 5.0 and below Pseudomonas oleovorans
6 11 30 min, purified enzyme, over 70% remaining activity Pseudomonas oleovorans