Cloned (Comment) | Organism |
---|---|
ORF ophc2, subcloning in Escherichia coli strain JM109, functional high level expression of OPHC2 in Pichia pastoris strain GS115, secretion to the culture supernatant | Pseudomonas oleovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5% inhibition at 1 mM | Pseudomonas oleovorans | |
Mn2+ | 18% inhibition at 1 mM | Pseudomonas oleovorans | |
Pb2+ | 20% inhibition at 1 mM | Pseudomonas oleovorans | |
Zn2+ | 11% inhibition at 1 mM | Pseudomonas oleovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates by 14.2% at 1 mM | Pseudomonas oleovorans | |
additional information | no effect by Ca2+, Cr3+, Mg2+, Li+, and EDTA at 1 mM | Pseudomonas oleovorans | |
Ni2+ | activates by 23.5% at 1 mM | Pseudomonas oleovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36000, recombinant OPHC2, SDS-PAGE | Pseudomonas oleovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas oleovorans | Q5W503 | ORF ophc2 | - |
Pseudomonas oleovorans C2-1 | Q5W503 | ORF ophc2 | - |
Purification (Comment) | Organism |
---|---|
recombinant OPHC2 from Pichia pastoris strain GS115 culture supernatant by gel filtration and ultrafiltration | Pseudomonas oleovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl parathion + H2O | - |
Pseudomonas oleovorans | dimethyl thiophosphate + 4-nitrophenol | - |
? | |
methyl parathion + H2O | - |
Pseudomonas oleovorans C2-1 | dimethyl thiophosphate + 4-nitrophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 36000, recombinant OPHC2, SDS-PAGE | Pseudomonas oleovorans |
Synonyms | Comment | Organism |
---|---|---|
OPHC2 | - |
Pseudomonas oleovorans |
organophosphorus hydrolase | - |
Pseudomonas oleovorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
recombinant enzyme | Pseudomonas oleovorans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | - |
Pseudomonas oleovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
30 min, purified recombinant enzyme, over 60% remaining activity | Pseudomonas oleovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
recombinant enzyme | Pseudomonas oleovorans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | 50% of maximal activity at pH 5.0 and below | Pseudomonas oleovorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
30 min, purified enzyme, loss of 50% activity at pH 5.0 and below | Pseudomonas oleovorans |
6 | 11 | 30 min, purified enzyme, over 70% remaining activity | Pseudomonas oleovorans |