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Literature summary for 3.1.8.1 extracted from
- In crystallo capture of a Michaelis complex and product-binding modes of a bacterial phosphotriesterase (2008), J. Mol. Biol., 375, 1189-1196.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Agrobacterium tumefaciens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic modelling, molecular dynamic docking studies and density functional theory calculations, overview | Agrobacterium tumefaciens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | the enzyme is a natively heterobinuclear ironzinc metalloprotein, binding structure, overview | Agrobacterium tumefaciens |  |
| additional information | the my-hydroxo bridge between the metal ions initiates the hydrolytic reaction, roles of alpha- and beta-metal ions in catalysis, overview | Agrobacterium tumefaciens | |
| Zn2+ | the enzyme is a natively heterobinuclear iron-zinc metalloprotein, binding structure, overview | Agrobacterium tumefaciens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium tumefaciens | Q93LD7 | gene opdA | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| an aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol | enzyme-substrate complex structure analysis and reaction mechanism, the my-hydroxo bridge between the metal ions initiates the hydrolytic reaction, roles of alpha- and beta-metal ions, contribution of grouns-state destabilization to catalysis, overview, Michaelis complex, transition state, and product structure, overview | Agrobacterium tumefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl parathion + H2O | i.e. O,O-dimethyl O-4-nitrophenyl phosphorothioate | Agrobacterium tumefaciens | dimethyl thiophosphate + 4-nitrophenol | - |
? | |
| additional information | substrate binding modes and structure, overview | Agrobacterium tumefaciens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| bacterial phosphotriesterase | - |
Agrobacterium tumefaciens |
| OpdA | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Agrobacterium tumefaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.1 | - |
assay at | Agrobacterium tumefaciens |
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Release 2023.1 (January 2023)
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