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Literature summary for 3.1.8.1 extracted from

  • Walker, A.W.; Keasling, J.D.
    Metabolic engineering of Pseudomonas putida for the utilization of parathion as a carbon and energy source. (2002), Biotechnol. Bioeng., 78, 715-721.
    View publication on PubMed
Search for more literature for 3.1.8.1

Cloned(Commentary)

Cloned (Comment) Organism
gene opd, expression of the gene with and without secretory leader sequence using the tac and taclac promoters Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information transformation of Pseudomnas putida with the opd gene and the 4-nitrophenyl PNP operon allows the engineered organism to utilize parathion as sole source of carbon and energy, overview Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 gene opd
-
Pseudomonas putida KT 2442
-
 gene opd
-

Source Tissue

Source Tissue Comment Organism Textmining
cell culture an engineered strain growing on parathion Pseudomonas putida
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
parathion + H2O engneered mutant strain, parathion metabolism, overview Pseudomonas putida 4-nitrophenol + diethyl thiophosphate
-
 ?
parathion + H2O engneered mutant strain, parathion metabolism, overview Pseudomonas putida KT 2442 4-nitrophenol + diethyl thiophosphate
-
 ?

Synonyms

Synonyms Comment Organism
OPH
-
 Pseudomonas putida
organophosphate hydrolase
-
 Pseudomonas putida