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Literature summary for 3.1.8.1 extracted from

  • Votchitseva, Y.A.; Efremenko, E.N.; Aliev, T.K.; Varfolomeyev, S.D.
    Properties of hexahistidine-tagged organophosphate hydrolase (2006), Biochemistry (Moscow), 71, 167-172.
    View publication on PubMed
Search for more literature for 3.1.8.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged OPH in Escherichia coli strains DH5alpha and SG13009, the His6-tagged enzyme shows altered properties compared to the wild-type enzyme Brevundimonas diminuta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Brevundimonas diminuta
0.01
-
 paraoxon pH 10.5, 20°C, recombinant His6-tagged OPH Brevundimonas diminuta
0.015
-
 parathion pH 10.5, 20°C, recombinant His6-tagged OPH Brevundimonas diminuta
0.016
-
 paraoxon pH 9.0, 20°C, native OPH Brevundimonas diminuta
0.06
-
 parathion pH 9.0, 20°C, native OPH Brevundimonas diminuta
0.073
-
 methylparathion pH 10.5, 20°C, recombinant His6-tagged OPH Brevundimonas diminuta
0.21
-
 methylparathion pH 9.0, 20°C, native OPH Brevundimonas diminuta

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates, preferred divalent metal ion by recombinant His6-OPH Brevundimonas diminuta
Cu2+ activates Brevundimonas diminuta
Mn2+ activates Brevundimonas diminuta
additional information requirement for divalent metal ions, the activity of recombinant His6-OPH decreases in the series: Co2+, Ni2+, Mn2+, Cu2+, Zn2+ Brevundimonas diminuta
Ni2+ activates Brevundimonas diminuta
Zn2+ activates Brevundimonas diminuta

Organism

Organism UniProt Comment Textmining
Brevundimonas diminuta
-
-
-
Brevundimonas diminuta VKM B-1297
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged OPH from Escherichia coli by nickel affinity chromatography Brevundimonas diminuta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl parathion + H2O
-
 Brevundimonas diminuta dimethyl thiophosphate + 4-nitrophenol
-
 ?
methyl parathion + H2O
-
 Brevundimonas diminuta VKM B-1297 dimethyl thiophosphate + 4-nitrophenol
-
 ?
paraoxon + H2O i.e. diethyl-4-nitrophenyl phosphate Brevundimonas diminuta diethylphosphate + 4-nitrophenol
-
 ?
paraoxon + H2O i.e. diethyl-4-nitrophenyl phosphate Brevundimonas diminuta VKM B-1297 diethylphosphate + 4-nitrophenol
-
 ?
parathion + H2O i.e. diethyl-4-nitrophenyl thiophosphate Brevundimonas diminuta diethylthiophosphate + 4-nitrophenol
-
 ?
parathion + H2O i.e. diethyl-4-nitrophenyl thiophosphate Brevundimonas diminuta VKM B-1297 diethylthiophosphate + 4-nitrophenol
-
 ?

Synonyms

Synonyms Comment Organism
OPH
-
 Brevundimonas diminuta
organophosphate hydrolase
-
 Brevundimonas diminuta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55 native OPH Brevundimonas diminuta
53 58 recombinant His6-tagged OPH Brevundimonas diminuta

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70
-
 Brevundimonas diminuta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 native OPH Brevundimonas diminuta
10.5
-
 recombinant His6-tagged OPH Brevundimonas diminuta

pH Range

pH Minimum pH Maximum Comment Organism
8.5 11.5
-
 Brevundimonas diminuta

pI Value

Organism Comment pI Value Maximum pI Value
Brevundimonas diminuta native OPH, isoelectric focusing
-
 7.4
Brevundimonas diminuta recombinant His6-tagged OPH, isoelectric focusing
-
 8.5