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Literature summary for 3.1.8.1 extracted from
- Purification of His6-organophosphate hydrolase using monolithic supermacroporous polyacrylamide cryogels developed for immobilized metal affinity chromatography (2006), Appl. Microbiol. Biotechnol., 70, 558-563.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged OPH in enzyme-deficient Escherichia coli strain SG13009 | Brevundimonas diminuta |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevundimonas diminuta | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged from Escherichia coli strain SG13009 OPH 29-43fold by agarose-based nickel affinity chromatography, and 60fold by affinity chromatography on divalent metal ion-iminodiacetic acidpolyacrylamide cryogel, usage of Ni2+, Co2+ or Cu2+ as methyl ions, method development and evaluation, effect of sample pretreatment on His6-OPH purification, overview | Brevundimonas diminuta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| paraoxon + H2O | i.e. diethyl-4-nitrophenyl phosphate | Brevundimonas diminuta | diethylphosphate + 4-nitrophenol | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| OPH | - |
Brevundimonas diminuta |
| organophosphate hydrolase | - |
Brevundimonas diminuta |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Brevundimonas diminuta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
assay at | Brevundimonas diminuta |
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