Literature summary for 3.1.8.1 extracted from

Aharoni, A.; Gaidukov, L.; Yagur, S.; Toker, L.; Silman, I.; Tawfik, D.S.
Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization (2004), Proc. Natl. Acad. Sci. USA, 101, 482-487.

Cloned(Commentary)

Cloned (Comment)	Organism
application of directed evolution in achieving functional expression of PON1 and PON3 in Escherichia coli culture and a dramatic increase in their hydrolytic proficiency toward the fluorogenic organophosphate substrate 7-O-diethylphosphoryl-3-cyano-7-hydroxycoumarin. The powerful tool of directed evolution opens new prospects for improving their phosphotriesterase activity toward other hazardous organophosphates and toward catalytic activities related to the prevention of atherosclerosis	Homo sapiens

Cloned (Comment)

Organism

application of directed evolution in achieving functional expression of PON1 and PON3 in Escherichia coli culture and a dramatic increase in their hydrolytic proficiency toward the fluorogenic organophosphate substrate 7-O-diethylphosphoryl-3-cyano-7-hydroxycoumarin. The powerful tool of directed evolution opens new prospects for improving their phosphotriesterase activity toward other hazardous organophosphates and toward catalytic activities related to the prevention of atherosclerosis

Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
0.085	-	paraoxon	pH 8.0, recombinant genetic variant G1A5	Homo sapiens
0.088	-	paraoxon	pH 8.0, recombinant genetic variant G3H8	Homo sapiens
0.089	-	paraoxon	pH 8.0, recombinant genetic variant G2E6	Homo sapiens
0.094	-	paraoxon	pH 8.0, recombinant genetic variant G3C9	Homo sapiens
0.1	-	paraoxon	pH 8.0, recombinant genetic variant G2D6	Homo sapiens
0.12	-	paraoxon	pH 8.0, recombinant genetic variant G1C4	Homo sapiens
0.51	-	paraoxon	pH 8.0, recombinant genetic variant G3A5	Homo sapiens
0.54	-	paraoxon	pH 8.0, wild-type enzyme	Homo sapiens
0.75	-	paraoxon	pH 8.0, recombinant genetic variant G3G3	Homo sapiens
0.8	-	paraoxon	pH 8.0, recombinant genetic variant G2C2	Homo sapiens
0.8	-	paraoxon	pH 8.0, recombinant genetic variant G3H9	Homo sapiens
1.1	-	paraoxon	pH 8.0, recombinant genetic variant G1B11	Homo sapiens
1.3	-	paraoxon	pH 8.0	Oryctolagus cuniculus
1.4	-	paraoxon	pH 8.0	Mus musculus
2.5	-	paraoxon	pH 8.0, recombinant genetic variant G1A7	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P27169	-	-
Homo sapiens	Q15166	-	-
Mus musculus	Q62087	-	-
Oryctolagus cuniculus	Q9BGN0	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
7-O-diethylphosphoryl-3-cyano-7-hydroxycoumarin + H2O	-	Homo sapiens	?	-	?
paraoxon + H2O	-	Homo sapiens	diethylphosphate + 4-nitrophenol	-	?
paraoxon + H2O	-	Homo sapiens	4-nitrophenol + diethyl phosphate	-	?
paraoxon + H2O	-	Oryctolagus cuniculus	4-nitrophenol + diethyl phosphate	-	?
paraoxon + H2O	-	Mus musculus	4-nitrophenol + diethyl phosphate	-	?
phenyl acetate + H2O	-	Homo sapiens	phenol + acetate	-	?

Synonyms

Synonyms	Comment	Organism
PON1	-	Homo sapiens
PON3	-	Homo sapiens
PON3	-	Oryctolagus cuniculus
PON3	-	Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.0008	-	paraoxon	pH 8.0	Mus musculus
0.001	-	paraoxon	pH 8.0	Oryctolagus cuniculus
0.007	-	paraoxon	pH 8.0, recombinant genetic variant G1A7	Homo sapiens
0.009	-	paraoxon	pH 8.0, recombinant genetic variant G1B11	Homo sapiens
0.036	-	paraoxon	pH 8.0, recombinant genetic variant G2C2	Homo sapiens
0.04	-	paraoxon	pH 8.0, recombinant genetic variant G3A5	Homo sapiens
0.11	-	paraoxon	pH 8.0, recombinant genetic variant G3G3	Homo sapiens
0.14	-	paraoxon	pH 8.0, recombinant genetic variant G3H9	Homo sapiens
0.54	-	paraoxon	pH 8.0, recombinant genetic variant G1C4	Homo sapiens
0.87	-	paraoxon	pH 8.0, recombinant genetic variant G2E6	Homo sapiens
0.98	-	paraoxon	pH 8.0, recombinant genetic variant G2D6	Homo sapiens
1.1	-	paraoxon	pH 8.0, recombinant genetic variant G3C9	Homo sapiens
1.16	-	paraoxon	pH 8.0, recombinant genetic variant G1A5	Homo sapiens
1.2	-	paraoxon	pH 8.0, recombinant genetic variant G3H8	Homo sapiens
3	-	paraoxon	pH 8.0, wild-type enzyme	Homo sapiens
552	-	phenyl acetate	pH 8.0, recombinant genetic variant G1C4	Homo sapiens
562	-	phenyl acetate	pH 8.0, recombinant genetic variant G2D6	Homo sapiens
789	-	phenyl acetate	pH 8.0, recombinant genetic variant G3C9	Homo sapiens
833	-	phenyl acetate	pH 8.0, recombinant genetic variant G1A5	Homo sapiens
965	-	phenyl acetate	pH 8.0, recombinant genetic variant G2E6	Homo sapiens
1018	-	phenyl acetate	pH 8.0, recombinant genetic variant G3H8	Homo sapiens
1236	-	phenyl acetate	pH 8.0, recombinant wild-type PON1	Homo sapiens