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Literature summary for 3.1.8.1 extracted from
- Improved degradation of organophosphorus nerve agents and p-nitrophenol by Pseudomonas putida JS444 with surface-expressed organophosphorus hydrolase (2005), Biotechnol. Prog., 21, 678-681.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | transformation of strain JS444 with a surface anchor system derive from Pseudomonas syringae leads to cell surface-taregeted expression of the enzyme reducing the limitations on substrate uptake, the engineered cells show improved degradation of organophosphorus pesticides and 4-nitrophenol, evaluation, overview | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pseudomonas putida | improved degradation of organophosphorus pesticides by the engineered cell surface-expressed PON1 of transformeted strain JS444 | ? | - |
? |  |
| additional information | Pseudomonas putida JS444 | improved degradation of organophosphorus pesticides by the engineered cell surface-expressed PON1 of transformeted strain JS444 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | - |
- |
- |
| Pseudomonas putida JS444 | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Pseudomonas putida | 4-nitrophenol + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Pseudomonas putida JS444 | 4-nitrophenol + phosphate | - |
? | |
| methyl parathion + H2O | - |
Pseudomonas putida | 4-nitrophenol + dimethyl thiophosphate | - |
? | |
| methyl parathion + H2O | - |
Pseudomonas putida JS444 | 4-nitrophenol + dimethyl thiophosphate | - |
? | |
| additional information | improved degradation of organophosphorus pesticides by the engineered cell surface-expressed PON1 of transformeted strain JS444 | Pseudomonas putida | ? | - |
? | |
| additional information | improved degradation of organophosphorus pesticides by the engineered cell surface-expressed PON1 of transformeted strain JS444 | Pseudomonas putida JS444 | ? | - |
? | |
| paraoxon + H2O | - |
Pseudomonas putida | 4-nitrophenol + diethyl phosphate | - |
? | |
| paraoxon + H2O | - |
Pseudomonas putida JS444 | 4-nitrophenol + diethyl phosphate | - |
? | |
| parathion + H2O | - |
Pseudomonas putida | 4-nitrophenol + diethyl thiophosphate | - |
? | |
| parathion + H2O | - |
Pseudomonas putida JS444 | 4-nitrophenol + diethyl thiophosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| organophosphorus hydrolase | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Pseudomonas putida |
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Release 2023.1 (January 2023)
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