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Literature summary for 3.1.8.1 extracted from
- Amphenicol and macrolide derived antibiotics inhibit paraoxonase enzyme activity in human serum and human hepatoma cells (HepG2) in vitro (2006), Biochemistry (Moscow), 71, 46-50.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chloramphenicol | 50% inhibition at 0.0309 mg/ml; inhibits the extracellular and the hepatoma cell enzyme, IC50, overview | Homo sapiens |  |
| clarithromycin | 50% inhibition at 5.121 mg/ml; inhibits the extracellular and the hepatoma cell enzyme, IC50, overview | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.5 | - |
paraoxon | serum enzyme, pH 8.0, 37°C | Homo sapiens | |
| 4.16 | - |
paraoxon | pH 8.0, 37°C | Homo sapiens | |
| 4.16 | - |
paraoxon | liver enzyme, pH 8.0, 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
x * 43000, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Homo sapiens | - |
isoform hPON1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
| native extracellular serum enzyme by ammonium sulfate fractionation and hydrophobic interaction chromatography on a L-tyrosine and 1-naphthylamine containing resin to homogeneity | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Hep-G2 cell | - |
Homo sapiens | - |
| Hep-G2 cell | hepatoma cell line | Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| serum | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 227.3 | - |
pH 8.0, 37°C | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
| paraoxon + H2O | - |
Homo sapiens | diethylphosphate + 4-nitrophenol | - |
? | |
| paraoxon + H2O | - |
Homo sapiens | 4-nitrophenol + diethyl phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| paraoxonase | - |
Homo sapiens |
| PON1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Homo sapiens |
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