Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 | Brevundimonas diminuta |
Protein Variants | Comment | Organism |
---|---|---|
D233A | site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview | Brevundimonas diminuta |
D233N | site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview | Brevundimonas diminuta |
D301A | site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview | Brevundimonas diminuta |
D301N | site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview | Brevundimonas diminuta |
H254A | site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview | Brevundimonas diminuta |
H254N | site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview | Brevundimonas diminuta |
Y309F | site-directed mutagenesis, structure and kinetics of the mutant enzyme with different bound metal ions compared to the wild-type enzyme, overview | Brevundimonas diminuta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of the Zn-enzyme and the Cd-enzyme, wild-type and mutant, kinetic model, overview | Brevundimonas diminuta | |
0.3 | - |
diethyl 4-chlorophenyl thiophosphate | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta | |
0.34 | - |
diethyl 4-nitrophenyl phosphate | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
0.5 | - |
diethyl 4-chlorophenyl thiophosphate | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
0.6 | - |
parathion | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
0.6 | - |
diethyl 4-chlorophenyl thiophosphate | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
0.74 | - |
diethyl 4-nitrophenyl phosphate | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
1.5 | - |
parathion | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
1.6 | - |
diethyl 4-chlorophenyl phosphate | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
1.7 | - |
parathion | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta | |
1.9 | - |
diethyl 4-nitrophenyl phosphate | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta | |
3.3 | - |
diethyl 4-chlorophenyl phosphate | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
7 | - |
diethyl 4-chlorophenyl phosphate | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | activates slightly | Brevundimonas diminuta | |
Zn2+ | the enzyme is a zinc metalloenzyme | Brevundimonas diminuta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevundimonas diminuta | P0A434 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Brevundimonas diminuta |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol | mechanism of hydrolysis of organophosphates by the bacterial phosphotriesterase, reaction modelling, kinetic model | Brevundimonas diminuta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diethyl 4-chlorophenyl phosphate + H2O | - |
Brevundimonas diminuta | 4-chlorophenol + diethyl phosphate | - |
? | |
diethyl 4-chlorophenyl thiophosphate + H2O | - |
Brevundimonas diminuta | 4-chlorophenol + diethyl thiophosphate | - |
? | |
diethyl 4-nitrophenyl phosphate + H2O | - |
Brevundimonas diminuta | 4-nitrophenol + diethyl phosphate | - |
? | |
parathion + H2O | - |
Brevundimonas diminuta | 4-nitrophenol + diethyl thiophosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphotriesterase | - |
Brevundimonas diminuta |
PTE | - |
Brevundimonas diminuta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Brevundimonas diminuta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
diethyl 4-chlorophenyl phosphate | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
0.23 | - |
diethyl 4-chlorophenyl phosphate | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta | |
0.36 | - |
diethyl 4-chlorophenyl phosphate | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
1.1 | - |
diethyl 4-chlorophenyl thiophosphate | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta | |
2.2 | - |
diethyl 4-chlorophenyl thiophosphate | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
2.4 | - |
diethyl 4-chlorophenyl thiophosphate | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
300 | - |
parathion | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
600 | - |
parathion | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta | |
720 | - |
parathion | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
2060 | - |
diethyl 4-nitrophenyl phosphate | recombinant wild-type enzyme bound to Cd2+ and Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
2300 | - |
diethyl 4-nitrophenyl phosphate | recombinant wild-type enzyme bound to Zn2+, pH 9.0, 30°C | Brevundimonas diminuta | |
2500 | - |
diethyl 4-nitrophenyl phosphate | recombinant wild-type enzyme bound to Cd2+, pH 9.0, 30°C | Brevundimonas diminuta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Brevundimonas diminuta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH profiles of wild-type and mutant enzymes substituted with different metals, overview | Brevundimonas diminuta |