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Literature summary for 3.1.8.1 extracted from
- A fluorogenic substrate for detection of organophosphatase activity (2004), Anal. Biochem., 327, 140-148.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PON1, expression of two naturally occurring allelic variant isozymes L55R192 PON1 and L55Q192 PON1 in Escherichia coli strain DH5alpha and in CHO cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Homo sapiens | |
| 0.091 | - |
7-diethylphospho-6,8-difluor-4-methylumbelliferyl | pH 8.0, 37°C, recombinant isozyme L55R192 PON1 | Homo sapiens |  |
| 0.2 | - |
7-diethylphospho-6,8-difluor-4-methylumbelliferyl | pH 8.0, 37°C, recombinant isozyme L55Q192 PON1 | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | added to optimized fluorescence assay | Homo sapiens | |
| NaCl | added to optimized fluorescence assay | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
gene PON1, two naturally occuring allelic variant isozymes L55R192 PON1 and L55Q192 PON1 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-diethylphospho-6,8-difluor-4-methylumbelliferyl + H2O | high level of hydrolysis of the fluorogenic substrate, fluorescence assay method optimization | Homo sapiens | diethylphosphate + 6,8-difluor-4-methylumbelliferol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| OPH | - |
Homo sapiens |
| organophosphorus hydrolase | - |
Homo sapiens |
| paraoxonase | - |
Homo sapiens |
| PON | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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