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Literature summary for 3.1.8.1 extracted from

  • Rodrigo, L.; Gil, F.; Hernandez, A.F.; Lopez, O.; Pla, A.
    Identification of paraoxonase 3 in rat liver microsomes: purification and biochemical properties (2003), Biochem. J., 376, 261-268.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Rattus norvegicus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q68FP2 male Wistar rats
-

Purification (Commentary)

Purification (Comment) Organism
the presence of 2.5 mM Ca2+ and 0.1% (w/v) Triton X-100 (as detergent) in the buffers throughout the purification procedure is essential for maintaining the activity of the enzyme. In the absence of calcium and Triton X-100, the enzyme activity is quickly lost Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
4°C, purified enzyme is quite stable, showing no decrease in specific activity after storage for 1 month Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
paraoxon + H2O
-
Rattus norvegicus 4-nitrophenol + diethyl phosphate
-
?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
paraoxonase 3
-
Rattus norvegicus
PON3
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 55 180 min, quite stable Rattus norvegicus
60
-
90 min, 50% loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 almost 100% activity is maintained during the incubation period of 180 min Rattus norvegicus