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Literature summary for 3.1.7.2 extracted from

  • Mechold, U.; Murphy, H.; Brown, L.; Cashel, M.
    Intramolecular regulation of the opposing (p)ppGpp catalytic activities of RelSeq, the Rel/Spo enzyme from Streptococcus equisimilis (2002), J. Bacteriol., 184, 2878-2888.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloning of relSeq fragments and the expression is regulated using pBAD vectors Streptococcus dysgalactiae subsp. equisimilis

Engineering

Protein Variants Comment Organism
additional information diverse deletion mutants show synthesis and degradation activities differing from those of full-length enzyme Streptococcus dysgalactiae subsp. equisimilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Streptococcus dysgalactiae subsp. equisimilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
SDS-PAGE Streptococcus dysgalactiae subsp. equisimilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ppGpp + H2O Streptococcus dysgalactiae subsp. equisimilis i.e. guanosine-3',5'-bis(diphosphate) Gpp + diphosphate i.e. guanosine 5'-diphosphate r
ppGpp + H2O Streptococcus dysgalactiae subsp. equisimilis H46A i.e. guanosine-3',5'-bis(diphosphate) Gpp + diphosphate i.e. guanosine 5'-diphosphate r

Organism

Organism UniProt Comment Textmining
Streptococcus dysgalactiae subsp. equisimilis
-
H46A
-
Streptococcus dysgalactiae subsp. equisimilis H46A
-
H46A
-

Purification (Commentary)

Purification (Comment) Organism
enzyme fragments Streptococcus dysgalactiae subsp. equisimilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
enzyme fragment NH 1-385 Streptococcus dysgalactiae subsp. equisimilis
0.35
-
enzyme fragment NCH Streptococcus dysgalactiae subsp. equisimilis
52
-
full-length enzyme NC 1-739 Streptococcus dysgalactiae subsp. equisimilis
0.46
-
enzyme fragment NH 1-24 Streptococcus dysgalactiae subsp. equisimilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ppGpp + H2O i.e. guanosine-3',5'-bis(diphosphate) Streptococcus dysgalactiae subsp. equisimilis Gpp + diphosphate i.e. guanosine 5'-diphosphate ?
ppGpp + H2O i.e. guanosine-3',5'-bis(diphosphate) Streptococcus dysgalactiae subsp. equisimilis Gpp + diphosphate i.e. guanosine 5'-diphosphate r
ppGpp + H2O i.e. guanosine-3',5'-bis(diphosphate) Streptococcus dysgalactiae subsp. equisimilis H46A Gpp + diphosphate i.e. guanosine 5'-diphosphate ?
ppGpp + H2O i.e. guanosine-3',5'-bis(diphosphate) Streptococcus dysgalactiae subsp. equisimilis H46A Gpp + diphosphate i.e. guanosine 5'-diphosphate r

Synonyms

Synonyms Comment Organism
RelSeq
-
Streptococcus dysgalactiae subsp. equisimilis