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show all sequences of 3.1.7.1

Testicular sterols. V. Preparation and partial purification of a microsomal prenol pyrophosphate pyrophophohydrolase

Tsai, S.C.; Gaylor, J.L.; J. Biol. Chem. 241, 4043-4050 (1966)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ATP
0.93 mM stimulates rate of hydrolysis
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
1-butanol
-
Rattus norvegicus
3',5'-cAMP
-
Rattus norvegicus
arsenite
-
Rattus norvegicus
Co2+
-
Rattus norvegicus
Cu2+
-
Rattus norvegicus
farnesoic acid
-
Rattus norvegicus
isopentenol-diphosphate
-
Rattus norvegicus
isopentenyl diphosphate
-
Rattus norvegicus
Mg2+
loss of about 88% of activity at 10 mM
Rattus norvegicus
Mn2+
-
Rattus norvegicus
additional information
-
Rattus norvegicus
NaF
-
Rattus norvegicus
Plasma albumin
-
Rattus norvegicus
Zn2+
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
1.2
farnesol-diphosphate
-
Rattus norvegicus
4
10
isopentenol-diphosphate
-
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
3 mM stimulates the testicular enzyme 4-10fold
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
prenyl diphosphate + H2O
Rattus norvegicus
-
prenol + diphosphate
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
125fold, partially purified
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
testis
-
Rattus norvegicus
-
Storage Stability
Storage Stability
Organism
frozen preperations retained activity for 1 month
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
farnesyl diphosphate + H2O
-
95169
Rattus norvegicus
farnesol + diphosphate
-
95169
Rattus norvegicus
?
glucose-6-phosphate + H2O
-
95169
Rattus norvegicus
glucose + phosphate
-
-
-
?
isopentenyl diphosphate + H2O
-
95169
Rattus norvegicus
isopentenol + diphosphate
-
95169
Rattus norvegicus
?
mevalonate-5-phosphate + H2O
-
95169
Rattus norvegicus
mevalonate + phosphate
-
-
-
?
additional information
-
95169
Rattus norvegicus
?
-
-
-
-
prenyl diphosphate + H2O
-
95169
Rattus norvegicus
prenol + diphosphate
-
-
-
-
prenyl diphosphate + H2O
-
95169
Rattus norvegicus
prenol + diphosphate
-
95169
Rattus norvegicus
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
enzyme in crude acetone powder
Rattus norvegicus
8.8
-
purified enzyme
Rattus norvegicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ATP
0.93 mM stimulates rate of hydrolysis
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1-butanol
-
Rattus norvegicus
3',5'-cAMP
-
Rattus norvegicus
arsenite
-
Rattus norvegicus
Co2+
-
Rattus norvegicus
Cu2+
-
Rattus norvegicus
farnesoic acid
-
Rattus norvegicus
isopentenol-diphosphate
-
Rattus norvegicus
isopentenyl diphosphate
-
Rattus norvegicus
Mg2+
loss of about 88% of activity at 10 mM
Rattus norvegicus
Mn2+
-
Rattus norvegicus
additional information
-
Rattus norvegicus
NaF
-
Rattus norvegicus
Plasma albumin
-
Rattus norvegicus
Zn2+
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
1.2
farnesol-diphosphate
-
Rattus norvegicus
4
10
isopentenol-diphosphate
-
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
3 mM stimulates the testicular enzyme 4-10fold
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
prenyl diphosphate + H2O
Rattus norvegicus
-
prenol + diphosphate
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
125fold, partially purified
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Rattus norvegicus
-
testis
-
Rattus norvegicus
-
Storage Stability (protein specific)
Storage Stability
Organism
frozen preperations retained activity for 1 month
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
farnesyl diphosphate + H2O
-
95169
Rattus norvegicus
farnesol + diphosphate
-
95169
Rattus norvegicus
?
glucose-6-phosphate + H2O
-
95169
Rattus norvegicus
glucose + phosphate
-
-
-
?
isopentenyl diphosphate + H2O
-
95169
Rattus norvegicus
isopentenol + diphosphate
-
95169
Rattus norvegicus
?
mevalonate-5-phosphate + H2O
-
95169
Rattus norvegicus
mevalonate + phosphate
-
-
-
?
additional information
-
95169
Rattus norvegicus
?
-
-
-
-
prenyl diphosphate + H2O
-
95169
Rattus norvegicus
prenol + diphosphate
-
-
-
-
prenyl diphosphate + H2O
-
95169
Rattus norvegicus
prenol + diphosphate
-
95169
Rattus norvegicus
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
enzyme in crude acetone powder
Rattus norvegicus
8.8
-
purified enzyme
Rattus norvegicus
Other publictions for EC 3.1.7.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
654930
Ha
-
Activities of soluble and micr ...
Datura stramonium
Biol. Plant.
47
477-479
2003
3
-
-
-
-
-
3
-
2
1
-
-
-
1
-
-
-
-
-
4
1
-
1
-
1
-
-
-
2
-
-
-
-
-
-
3
-
-
-
-
-
-
-
3
-
-
2
1
-
-
-
-
-
-
-
4
1
-
1
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
656974
Nah
Partial characterization of fa ...
Oryza sativa
Plant Cell Physiol.
42
864-867
2001
1
-
-
-
-
-
4
-
2
1
-
1
-
2
-
-
-
-
-
1
1
-
3
-
1
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
-
2
1
-
1
-
-
-
-
-
1
1
-
3
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
95168
Perez
-
Hydrolysis of allylic phosphat ...
Citrus sinensis
Phytochemistry
19
183-187
1980
-
-
-
-
-
-
7
-
-
-
-
1
-
1
-
-
1
-
-
1
-
2
11
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
1
-
-
-
1
-
1
-
2
11
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
95169
Tsai
Testicular sterols. V. Prepara ...
Rattus norvegicus
J. Biol. Chem.
241
4043-4050
1966
1
-
-
-
-
-
14
2
1
1
-
1
-
1
-
-
1
-
-
2
-
1
7
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
14
-
2
1
1
-
1
-
-
-
1
-
2
-
1
7
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-