Application | Comment | Organism |
---|---|---|
molecular biology | Ars can be very useful for clarifying the mechanisms underpinning syndromes caused by the deficiency of the function of Ars genes | Mus musculus |
Cloned (Comment) | Organism |
---|---|
recombinant expression of myc-His- or GFP-tagged ArsA, the recombinant protein is secreted into the serum-free medium | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | coating culture plates with recombinant mouse ArsA stimulates adhesion of human microvascular endothelial cells to the plate followed by the formation of cell protrusions as well as lamellipodia | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | a large portion of the mammalian arylsulfatase A protein exists on the cell surface of vascular endothelial cells | Mus musculus | 9986 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | recombinant mouse ArsA tightly binds to sulfated polysaccharides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P50428 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant myc-His- or GFP-tagged ArsA by affinity chromatography from culture cell supernatant | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
vascular endothelial cell | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | recombinant mouse ArsA tightly binds to sulfated polysaccharides | Mus musculus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the polypeptide complexes, rather than the monomers, are subject to endoplasmic reticulum quality control and, within a heteromer, the misfolded subunit exerts a dominant negative effect on the wild-type subunit | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
ARS | - |
Mus musculus |
ARSA | - |
Mus musculus |
arylsulfatase A | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | inherited deficiency of arylsulfatase A leads to metachromatic leukodystrophy, MLD, a disease involving demyelination in the central and peripheral nervous systems, with an estimated frequency of 1 in 40000 newborns. Inherited deficiency for arylsulfatase leads to lysosomal storage of sulfated compounds and to serious diseases such as growth retardation, heart failure, and demyelination in the central nervous system | Mus musculus |
metabolism | ArsA is involved in the modulation of a sort of signaling cascade, which affects the morphology of the cells, by binding to the HSPGs located on the cell surface | Mus musculus |
physiological function | ArsA plays a role in the components of the extracellular matrix. ArsA functions as a substrate on which cells adhere and form protrusions. Coating culture plates with recombinant mouse ArsA, rmArsA, stimulates adhesion of human microvascular endothelial cells to the plate followed by the formation of cell protrusions as well as lamellipodia. rmArsA affects the architecture of the cytoskeleton, with a high density of actin filaments localized to peripheral regions of the cells and the extension of bundles of microtubules into the tips of cellular protrusions. rmArsA also affects the distribution pattern of the cell adhesion-associated proteins, integrin alpha2beta1, and paxillin. rmArsA seems to modulate signaling of basic fibroblast growth factor stimulating cytoskeletal rearrangement | Mus musculus |