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Literature summary for 3.1.6.8 extracted from

  • von Bulow, R.; Schmidt, B.; Dierks, T.; von Figura, K.; Uson, I.
    Crystal structure of an enzyme-substrate complex provides insight into the interaction between human arylsulfatase A and its substrates during catalysis (2001), J. Mol. Biol., 305, 269-277.
    View publication on PubMed
Search for more literature for 3.1.6.8

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of mutant C69A a nd C69S in complex with p-nitrocatechol sulfate Homo sapiens
crystal structure of mutant C69A in complex with p-nitrocatechol sulfate, Protein Data Bank: 1E1Z and 1E2S, and crystal structure of mutant C69S in complex with p-nitrocatechol sulfate, Protein Data Bank: 1E33 and 1E3C Homo sapiens

Protein Variants

Protein Variants Comment Organism
C69A The inactive mutant in complex with p-nitrocatechol sulfate mimics a reaction intermediate during sulfate ester hydrolysis by the active enzyme, without the covalent bond to the key side-chain C-alpha-formylglycine Homo sapiens
C69S the mutant is able to bind covalently to the substrate and hydrolyse it, but is unable to release the resulting sulfate Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cerebroside 3-sulfate + H2O Homo sapiens
-
 cerebroside + sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P15289
-
-

Reaction

Reaction Comment Organism Reaction ID
a cerebroside 3-sulfate + H2O = a cerebroside + sulfate A detailed mechanism for sulfate ester cleavage is proposed, involving an aldehyde hydrate as the functional group. Homo sapiens
a
a cerebroside 3-sulfate + H2O = a cerebroside + sulfate a detailed mechanism for sulfate ester cleavage is proposed, involving an aldehyde hydrate as the functional group Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cerebroside 3-sulfate + H2O
-
 Homo sapiens cerebroside + sulfate
-
 ?
p-nitrocatechol sulfate + H2O
-
 Homo sapiens p-nitrocatechol + sulfate
-
 ?