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Literature summary for 3.1.6.8 extracted from

  • Stevens, R.L.; Fluharty, A.L.; Skokut, M.H.; Kihara, H.
    Purification and properties of arylsulfatase. A from human urine (1975), J. Biol. Chem., 250, 2495-2501.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
taurodeoxycholate enzyme cleaves cerebroside sulfates only in the presence of either specific detergent, e.g. taurodeoxycholate or human activator protein Homo sapiens

General Stability

General Stability Organism
bovine serum albumin stabilizes Homo sapiens
4°C, addition of dithiothreitol, glutathione, or EDTA reduce storage stability Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
cerebroside 3-sulfate
-
Homo sapiens
8
-
4-Methylumbelliferyl sulfate
-
Homo sapiens
6.7
-
4-nitrocatechol sulfate
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ stimulates Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
SDS-PAGE Homo sapiens
100000
-
sedimentation equilibrium ultracentrifugation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cerebroside 3-sulfate + H2O Homo sapiens
-
cerebroside + sulfate
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
urine
-
Homo sapiens
-

Storage Stability

Storage Stability Organism
-20°C, protein: 1 mg/ml, stable for months Homo sapiens
4°C, pH 7.5, protein: 0.002 mg/ml, bovine serum albumin, insulin or ribonuclease, 15 days, no loss of activity Homo sapiens
4°C, pH 7.5, protein: 0.002 mg/ml, bovine serum albumin, insulin or ribonuclease, 8 months, 30% loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methylumbelliferyl sulfate + H2O
-
Homo sapiens methylumbelliferone + sulfate
-
?
cerebroside 3-sulfate + H2O
-
Homo sapiens cerebroside + sulfate
-
?

Subunits

Subunits Comment Organism
dimer 2 * ca. 55000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16.8
-
4-nitrocatechol sulfate
-
Homo sapiens
1.45
-
cerebroside 3-sulfate
-
Homo sapiens
0.817
-
4-Methylumbelliferyl sulfate
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3
-
4-nitrocatechol sulfate Homo sapiens
5.2
-
4-methylumbelliferyl sulfate Homo sapiens
4.6
-
cerebroside sulfate Homo sapiens