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Literature summary for 3.1.6.6 extracted from
- Choline sulfatase from Ensifer (Sinorhizobium) meliloti Characterization of the unmodified enzyme (2015), Biochem. Biophys. Rep., 3, 161-168 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Sinorhizobium meliloti |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| choline | competitive with respect to choline sulfate | Sinorhizobium meliloti |  |
| Choline sulfate | substrate inhibition | Sinorhizobium meliloti | |
| additional information | not inhibitory: ammonium sulfate up to 70 mM | Sinorhizobium meliloti |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.1 | - |
Choline sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
| 11.8 | - |
4-Methylumbelliferyl sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
| 75.8 | - |
4-nitrophenyl sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 118000 | - |
MALDI-TOF | Sinorhizobium meliloti |
| 123000 | - |
gel filtration | Sinorhizobium meliloti |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sinorhizobium meliloti | O69787 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | absence of formylglycine posttranslational modification in active-site cysteine54 | Sinorhizobium meliloti |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl sulfate + H2O | - |
Sinorhizobium meliloti | methylumbelliferone + sulfate | - |
? | |
| 4-nitrophenyl sulfate + H2O | - |
Sinorhizobium meliloti | 4-nitrophenol + sulfate | - |
? | |
| choline sulfate + H2O | - |
Sinorhizobium meliloti | choline + sulfate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 63000, SDS-PAGE, recombinant protein including His-tag, 2 * 59000, MALDI-TOF | Sinorhizobium meliloti |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.035 | - |
4-nitrophenyl sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
| 0.043 | - |
4-Methylumbelliferyl sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
| 0.27 | - |
Choline sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Sinorhizobium meliloti |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.4 | - |
Choline sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
| 57.8 | - |
choline | pH 7.5, 25°C | Sinorhizobium meliloti | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00046 | - |
4-nitrophenyl sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
| 0.0036 | - |
4-Methylumbelliferyl sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
| 0.0243 | - |
Choline sulfate | pH 7.5, 25°C | Sinorhizobium meliloti | |
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Release 2023.1 (January 2023)
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