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Literature summary for 3.1.6.6 extracted from
- Choline sulfatase of Pseudomonas aeruginosa (1972), Arch. Biochem. Biophys., 153, 664-672.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Choline sulfate | - |
Pseudomonas aeruginosa |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| betaine | linear competitive inhibitor | Pseudomonas aeruginosa | |
| choline | partial competitive inhibitor | Penicillium chrysogenum | |
| choline | uncompetitive inhibitor | Pseudomonas aeruginosa | |
| Dimethylamine | uncompetitive inhibitor | Pseudomonas aeruginosa | |
| dimethylaminoethanol | uncompetitive inhibitor | Pseudomonas aeruginosa | |
| dithiothreitol | - |
Penicillium chrysogenum | |
| dithiothreitol | after preincubation | Pseudomonas aeruginosa | |
| mercaptoethanol | - |
Penicillium chrysogenum | |
| mercaptoethanol | after preincubation | Pseudomonas aeruginosa | |
| methionine | synthesis of the enzyme is repressed | Pseudomonas aeruginosa | |
| N-ethylmaleimide | - |
Penicillium chrysogenum | |
| N-ethylmaleimide | - |
Pseudomonas aeruginosa | |
| p-chloromercuribenzoate | - |
Penicillium chrysogenum | |
| p-chloromercuribenzoate | - |
Pseudomonas aeruginosa | |
| phosphorylcholine | competitive inhibition | Pseudomonas aeruginosa | |
| tetramethylamine | uncompetitive inhibitor | Pseudomonas aeruginosa | |
| trimethylamine | uncompetitive inhibitor | Pseudomonas aeruginosa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
Choline sulfate | - |
Pseudomonas aeruginosa | |
| 8.5 | - |
Choline sulfate | - |
Penicillium chrysogenum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | complete inhibition | Pseudomonas aeruginosa | |
| Cd2+ | complete inhibition | Pseudomonas aeruginosa | |
| Co2+ | complete inhibition | Pseudomonas aeruginosa | |
| Cu2+ | complete inhibition | Pseudomonas aeruginosa | |
| Mn2+ | complete inhibition | Pseudomonas aeruginosa | |
| Zn2+ | complete inhibition | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 175000 | - |
gel filtration | Pseudomonas aeruginosa |
| 345000 | - |
gel filtration | Penicillium chrysogenum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choline sulfate + H2O | Penicillium chrysogenum | - |
choline + sulfate | - |
? | |
| choline sulfate + H2O | Pseudomonas aeruginosa | substrate-induced | choline + sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium chrysogenum | - |
- |
- |
| Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Pseudomonas aeruginosa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| choline sulfate + H2O = choline + sulfate | ordered bi-bi mechanism | Penicillium chrysogenum | |
| choline sulfate + H2O = choline + sulfate | ordered bi-bi mechanism | Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.262 | - |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| choline sulfate + H2O | - |
Penicillium chrysogenum | choline + sulfate | - |
? | |
| choline sulfate + H2O | reverse reaction not demonstrated | Penicillium chrysogenum | choline + sulfate | - |
ir | |
| choline sulfate + H2O | reverse reaction not demonstrated | Pseudomonas aeruginosa | choline + sulfate | - |
ir | |
| choline sulfate + H2O | substrate-induced | Pseudomonas aeruginosa | choline + sulfate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Pseudomonas aeruginosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Pseudomonas aeruginosa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Pseudomonas aeruginosa |
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