Application | Comment | Organism |
---|---|---|
drug development | HIRMAb-IDS fusion protein is a bifunctional IgG-sulfatase fusion protein, specifically engineered for targeted drug delivery across the human blood-brain barrier | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
HIRMAb-IDS fusion protein (IDS cDNA, encoding Ser26-Pro550, minus the 25 amino acid signal peptide, fused to the carboxyl terminus of the heavy chain of a chimeric monoclonal antibody to the human insulin receptor) expressed in COS cells | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the fusion protein is about 80 kDa larger than the heavy chain of the chimeric HIRMAb | Homo sapiens |
108000 | - |
heavy-chain HIRMAb-IDS fusion protein, calculated from sequence | Homo sapiens |
135000 | - |
heavy-chain HIRMAb-IDS fusion protein, Western blotting | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HIRMAb-IDS fusion protein purified by protein A affinity chromatography, to homogeneity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-alpha-L-iduronide-2-sulfate + H2O | - |
Homo sapiens | 4-methylumbelliferyl-alpha-L-iduronide + sulfate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | HIRMAb-IDS fusion protein | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
IDS | - |
Homo sapiens |
iduronate-2-sulfatase | - |
Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | heavy-chain HIRMAb-IDS fusion protein, calculated from sequence | - |
6.03 |
General Information | Comment | Organism |
---|---|---|
physiological function | HIRMAb-IDS fusion protein crosses the blood-brain barrier on the endogenous insulin receptor and acts as a molecular Trojan horse to ferry the IDS into brain. The fusion protein is taken up by Hunter fibroblasts, and the accumulation of glycosoaminoglycans in fibroblasts null for the sulfatase is decreased 84% by treatment with the fusion protein. The fusion protein heavy chain reacts with antibodies to both human IgG and human IDS. The fusion protein is a bifunctional molecule and binds both the HIR extracellular domain with comparable affinity to the chimeric HIRMAb and has high IDS enzyme activity | Homo sapiens |